Cryo-electron microscopy analysis of small membrane proteins
Highlights•Cryo-EM is a powerful tool to investigate the structures of membrane
proteins.•Recent technological advancements in the field of cryo-EM have enabled structure …
proteins.•Recent technological advancements in the field of cryo-EM have enabled structure …
Advancing the field of viroporins—Structure, function and pharmacology: IUPHAR Review 39
Viroporins possess important potential as antiviral targets due to their critical roles during
virus life cycles, spanning from virus entry to egress. Although the antiviral amantadine …
virus life cycles, spanning from virus entry to egress. Although the antiviral amantadine …
Routine sub-2.5 Å cryo-EM structure determination of GPCRs
Cryo-electron microscopy (cryo-EM) of small membrane proteins, such as G protein-coupled
receptors (GPCRs), remains challenging. Pushing the performance boundaries of the …
receptors (GPCRs), remains challenging. Pushing the performance boundaries of the …
[HTML][HTML] Cryo-EM studies of membrane proteins at 200 keV
Single-particle cryogenic electron-microscopy (cryo-EM) has emerged as a powerful
technique for the structural characterisation of membrane proteins, especially for targets …
technique for the structural characterisation of membrane proteins, especially for targets …
Imaging conformations of holo- and apo-transferrin on the single-molecule level by low-energy electron holography
Conformational changes play a key role in the biological function of many proteins, thereby
sustaining a multitude of processes essential to life. Thus, the imaging of the conformational …
sustaining a multitude of processes essential to life. Thus, the imaging of the conformational …
Molecular dynamics to predict cryo-EM: capturing transitions and short-lived conformational states of biomolecules
Single-particle cryogenic electron microscopy (cryo-EM) has revolutionized the field of the
structural biology, providing an access to the atomic resolution structures of large …
structural biology, providing an access to the atomic resolution structures of large …
An all-atom model of the human cardiac sodium channel in a lipid bilayer
GM Knotts, SK Lile, EM Campbell, TA Agee… - Scientific Reports, 2024 - nature.com
Voltage-gated sodium channels (NaV) are complex macromolecular proteins that are
responsible for the initial upstroke of an action potential in excitable cells. Appropriate …
responsible for the initial upstroke of an action potential in excitable cells. Appropriate …
Low-energy electron holography imaging of conformational variability of single-antibody molecules from electrospray ion beam deposition
Imaging of proteins at the single-molecule level can reveal conformational variability, which
is essential for the understanding of biomolecules. To this end, a biologically relevant state …
is essential for the understanding of biomolecules. To this end, a biologically relevant state …
Establishing the catalytic and regulatory mechanism of RNA‐based machineries
Ribonucleoprotein (RNP)‐machineries are comprised of intricate networks of long
noncoding RNAs and proteins that allow them to actively participate in transcription, RNA …
noncoding RNAs and proteins that allow them to actively participate in transcription, RNA …
Automated pipelines for rapid evaluation during cryoEM data acquisition
Cryo-electron microscopy (cryoEM) has become a popular method for determining high-
resolution structures of biomolecules. However, data processing can be time-consuming …
resolution structures of biomolecules. However, data processing can be time-consuming …