A programmable encapsulation system improves delivery of therapeutic bacteria in mice

T Harimoto, J Hahn, YY Chen, J Im, J Zhang… - Nature …, 2022 - nature.com
Living bacteria therapies have been proposed as an alternative approach to treating a broad
array of cancers. In this study, we developed a genetically encoded microbial encapsulation …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Guidelines for the use of flow cytometry and cell sorting in immunological studies*

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2017 - Wiley Online Library
From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

Antibiotic-induced changes to the host metabolic environment inhibit drug efficacy and alter immune function

JH Yang, P Bhargava, D McCloskey, N Mao… - Cell host & …, 2017 - cell.com
Bactericidal antibiotics alter microbial metabolism as part of their lethality and can damage
mitochondria in mammalian cells. In addition, antibiotic susceptibility is sensitive to …

Activation of caspase-1 by the NLRP3 inflammasome regulates the NADPH oxidase NOX2 to control phagosome function

A Sokolovska, CE Becker, WKE Ip, VAK Rathinam… - Nature …, 2013 - nature.com
Phagocytosis is a fundamental cellular process that is pivotal for immunity as it coordinates
microbial killing, innate immune activation and antigen presentation. An essential step in this …

Biofilm vs. planktonic bacterial mode of growth: which do human macrophages prefer?

E Hernández-Jiménez, R Del Campo… - Biochemical and …, 2013 - Elsevier
Although the natural mode of bacterial growth in nature is as biofilm, almost all antimicrobial
and immunological tests are routinely developed using planktonic inoculums. Bacterial …

KPC-producing, multidrug-resistant Klebsiella pneumoniae sequence type 258 as a typical opportunistic pathogen

LS Tzouvelekis, V Miriagou, SD Kotsakis… - Antimicrobial agents …, 2013 - journals.asm.org
The virulence of a KPC-producing Klebsiella pneumoniae sequence type 258 (ST258) strain
representing those circulating in Greece was assessed in a mouse septicemia model. The …

STAT2 signaling regulates macrophage phenotype during influenza and bacterial super-infection

R Gopal, B Lee, KJ McHugh, HE Rich… - Frontiers in …, 2018 - frontiersin.org
Influenza is a common respiratory virus that infects between 5 and 20% of the US population
and results in 30,000 deaths annually. A primary cause of influenza-associated death is …

Alternative enzyme protection assay to overcome the drawbacks of the gentamicin protection assay for measuring entry and intracellular survival of staphylococci

JH Kim, AK Chaurasia, N Batool, KS Ko… - Infection and …, 2019 - journals.asm.org
Precise enumeration of living intracellular bacteria is the key step to estimate the invasion
potential of pathogens and host immune responses to understand the mechanism and …