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A programmable encapsulation system improves delivery of therapeutic bacteria in mice
Living bacteria therapies have been proposed as an alternative approach to treating a broad
array of cancers. In this study, we developed a genetically encoded microbial encapsulation …
array of cancers. In this study, we developed a genetically encoded microbial encapsulation …
Guidelines for the use of flow cytometry and cell sorting in immunological studies
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …
immunology and flow cytometry community. They provide the theory and key practical …
Guidelines for the use of flow cytometry and cell sorting in immunological studies*
From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …
Antibiotic-induced changes to the host metabolic environment inhibit drug efficacy and alter immune function
Bactericidal antibiotics alter microbial metabolism as part of their lethality and can damage
mitochondria in mammalian cells. In addition, antibiotic susceptibility is sensitive to …
mitochondria in mammalian cells. In addition, antibiotic susceptibility is sensitive to …
Activation of caspase-1 by the NLRP3 inflammasome regulates the NADPH oxidase NOX2 to control phagosome function
Phagocytosis is a fundamental cellular process that is pivotal for immunity as it coordinates
microbial killing, innate immune activation and antigen presentation. An essential step in this …
microbial killing, innate immune activation and antigen presentation. An essential step in this …
Biofilm vs. planktonic bacterial mode of growth: which do human macrophages prefer?
Although the natural mode of bacterial growth in nature is as biofilm, almost all antimicrobial
and immunological tests are routinely developed using planktonic inoculums. Bacterial …
and immunological tests are routinely developed using planktonic inoculums. Bacterial …
KPC-producing, multidrug-resistant Klebsiella pneumoniae sequence type 258 as a typical opportunistic pathogen
The virulence of a KPC-producing Klebsiella pneumoniae sequence type 258 (ST258) strain
representing those circulating in Greece was assessed in a mouse septicemia model. The …
representing those circulating in Greece was assessed in a mouse septicemia model. The …
Deep-learning image analysis for high-throughput screening of opsono-phagocytosis-promoting monoclonal antibodies against Neisseria gonorrhoeae
Antimicrobial resistance (AMR) is nowadays a global health concern as bacterial pathogens
are increasingly develo** resistance to antibiotics. Monoclonal antibodies (mAbs) …
are increasingly develo** resistance to antibiotics. Monoclonal antibodies (mAbs) …
STAT2 signaling regulates macrophage phenotype during influenza and bacterial super-infection
Influenza is a common respiratory virus that infects between 5 and 20% of the US population
and results in 30,000 deaths annually. A primary cause of influenza-associated death is …
and results in 30,000 deaths annually. A primary cause of influenza-associated death is …
Alternative enzyme protection assay to overcome the drawbacks of the gentamicin protection assay for measuring entry and intracellular survival of staphylococci
Precise enumeration of living intracellular bacteria is the key step to estimate the invasion
potential of pathogens and host immune responses to understand the mechanism and …
potential of pathogens and host immune responses to understand the mechanism and …