Heterotrimeric guanine-nucleotide-binding regulatory proteins (G-proteins) mainly relay the
information from G-protein-coupled receptors (GPCRs) on the plasma membrane to the …

Cell-free systems (CFS) have recently evolved into key platforms for synthetic biology
applications. Many synthetic biology tools have traditionally relied on cell-based systems …

Mini-G proteins are the engineered GTPase domains of Gα subunits. They couple to GPCRs
and recapitulate the increase in agonist affinity observed upon coupling of a native …

Members of the heterotrimeric guanine nucleotide–binding protein (G protein)–coupled
receptor (GPCR) family play key roles in many physiological functions and are extensively …

The G-protein-coupled receptor (GPCR) signaling system is one of the main signaling
pathways in eukaryotes. Here, we analyze the evolutionary history of all its components …

Cell-free protein expression plays an important role in biochemical research. However, only
recent developments led to new methods to rapidly synthesize preparative amounts of …

Constitutively active G protein α subunits cause cancer, cholera, Sturge-Weber syndrome,
and other disorders. Therapeutic intervention by targeted inhibition of constitutively active …

Bone marrow stromal cells maintain the adult skeleton by forming osteoblasts throughout life
that regenerate bone and repair fractures. We discovered that subsets of these stromal cells …

Prior studies have shown that aversive olfactory memory is acquired by dopamine acting on
a specific receptor, dDA1, expressed by mushroom body neurons. Active forgetting is …

In the last few years the interactome of Gαq has expanded considerably, contributing to
improve our understanding of the cellular and physiological events controlled by this G …