Unlocking the promise of mRNA therapeutics

E Rohner, R Yang, KS Foo, A Goedel… - Nature biotechnology, 2022 - nature.com
The extraordinary success of mRNA vaccines against coronavirus disease 2019 (COVID-19)
has renewed interest in mRNA as a means of delivering therapeutic proteins. Early clinical …

Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading

J Rädler, D Gupta, A Zickler, SEL Andaloussi - Molecular Therapy, 2023 - cell.com
Extracellular vesicles (EVs) are gaining increasing attention for diagnostic and therapeutic
applications in various diseases. These natural nanoparticles benefit from favorable safety …

Identification of storage conditions stabilizing extracellular vesicles preparations

A Görgens, G Corso, DW Hagey… - Journal of …, 2022 - Wiley Online Library
Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological
processes and hold great potential for therapeutic and diagnostic use. Despite significant …

CD63 sorts cholesterol into endosomes for storage and distribution via exosomes

R Palmulli, M Couty, MC Piontek, M Ponnaiah… - Nature Cell …, 2024 - nature.com
Extracellular vesicles such as exosomes are now recognized as key players in intercellular
communication. Their role is influenced by the specific repertoires of proteins and lipids …

Antibody-displaying extracellular vesicles for targeted cancer therapy

OPB Wiklander, DR Mamand, DK Mohammad… - Nature biomedical …, 2024 - nature.com
Extracellular vesicles (EVs) function as natural delivery vectors and mediators of biological
signals across tissues. Here, by leveraging these functionalities, we show that EVs …

Targeted therapy using engineered extracellular vesicles: principles and strategies for membrane modification

Q Liu, D Li, X Pan, Y Liang - Journal of Nanobiotechnology, 2023 - Springer
Abstract Extracellular vesicles (EVs) are 30–150 nm membrane-bound vesicles naturally
secreted by cells and play important roles in intercellular communication by delivering …

The cellular response to extracellular vesicles is dependent on their cell source and dose

DW Hagey, M Ojansivu, BR Bostancioglu, O Saher… - Science …, 2023 - science.org
Extracellular vesicles (EVs) have been established to play important roles in cell-cell
communication and shown promise as therapeutic agents. However, we still lack a basic …

Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles

W Zheng, J Rädler, H Sork, Z Niu, S Roudi… - Nature …, 2023 - nature.com
Extracellular vesicles (EVs) are gaining ground as next-generation drug delivery modalities.
Genetic fusion of the protein of interest to a scaffold protein with high EV-sorting ability …

Cell-derived extracellular vesicles for CRISPR/Cas9 delivery: engineering strategies for cargo packaging and loading

Y Liang, Z Iqbal, J Wang, L Xu, X Xu, K Ouyang… - Biomaterials …, 2022 - pubs.rsc.org
Genome editing technology has emerged as a potential therapeutic tool for treating
incurable diseases. In particular, the discovery of clustered regularly interspaced short …

Exploring the clinical transition of engineered exosomes designed for intracellular delivery of therapeutic proteins

M Kim, H Choi, DJ Jang, HJ Kim, Y Sub… - Stem cells …, 2024 - academic.oup.com
Extracellular vesicles, particularly exosomes, have emerged as promising drug delivery
systems owing to their unique advantages, such as biocompatibility, immune tolerability, and …