A comprehensive understanding of host–pathogen interactions requires a knowledge of the
associated gene expression changes in both the pathogen and the host. Traditional, probe …

In trypanosomes, RNA polymerase II transcription is polycistronic and individual mRNAs are
excised by trans-splicing and polyadenylation. The lack of individual gene transcription …

Many evolutionarily distant pathogenic organisms have evolved similar survival strategies to
evade the immune responses of their hosts. These include antigenic variation, through …

Unicellular flagellates of the family Trypanosomatidae are obligatory parasites of
invertebrates, vertebrates and plants. Dixenous species are aetiological agents of a number …

One of the first steps in understanding a protein's function is to determine its localization;
however, the methods for localizing proteins in some systems have not kept pace with the …

The genome of Trypanosoma brucei, the causative agent of African trypanosomiasis, was
published five years ago, yet identification of all genes and their transcripts remains to be …

The kinetoplastid parasite, Trypanosoma brucei, undergoes a complex life cycle entailing
slender and stumpy bloodstream forms in mammals and procyclic and metacyclic forms …

Variant surface glycoprotein (VSG) coats bloodstream form Trypanosoma brucei parasites,
and monoallelic VSG expression underpins the antigenic variation necessary for …

The African trypanosome, Trypanosoma brucei, maintains an integral link between cell cycle
regulation and differentiation during its intricate life cycle. Whilst extensive changes in …

In trypanosomes, all RNAs are processed by the concerted action of trans-splicing and
polyadenylation. In trans-splicing, a common spliced leader (SL) is donated to all mRNAs …