Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
Step** on the gas to a circular economy: accelerating development of carbon-negative chemical production from gas fermentation
Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change
poses significant environmental, economic, and social challenges globally. Technologies …
poses significant environmental, economic, and social challenges globally. Technologies …
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery
The emerging discipline of bacterial glycoengineering has made it possible to produce
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
designer glycans and glycoconjugates for use as vaccines and therapeutics. Unfortunately …
Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor
Advances in biosensor engineering have enabled the design of programmable molecular
systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer …
systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer …
Enabling technology and core theory of synthetic biology
Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …
The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform
D Garenne, S Thompson, A Brisson… - Synthetic …, 2021 - academic.oup.com
The new generation of cell-free gene expression systems enables the prototy** and
engineering of biological systems in vitro over a remarkable scope of applications and …
engineering of biological systems in vitro over a remarkable scope of applications and …
Bottom-up construction of complex biomolecular systems with cell-free synthetic biology
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
An automated cell-free workflow for transcription factor engineering
The design and optimization of metabolic pathways, genetic systems, and engineered
proteins rely on high-throughput assays to streamline design-build-test-learn cycles …
proteins rely on high-throughput assays to streamline design-build-test-learn cycles …