Advances in genome editing through control of DNA repair pathways
Eukaryotic cells deploy overlap** repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …
Advancements in genome editing take advantage of these pathways to produce permanent …
CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …
However, the currently most practicable approaches rely on the generation of DNA double …
Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
R Hajian, S Balderston, T Tran, T DeBoer… - Nature biomedical …, 2019 - nature.com
Most methods for the detection of nucleic acids require many reagents and expensive and
bulky instrumentation. Here, we report the development and testing of a graphene-based …
bulky instrumentation. Here, we report the development and testing of a graphene-based …
Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair
J Ferreira da Silva, GP Oliveira, EA Arasa-Verge… - Nature …, 2022 - nature.com
Prime editing (PE) is a powerful genome engineering approach that enables the introduction
of base substitutions, insertions and deletions into any given genomic locus. However, the …
of base substitutions, insertions and deletions into any given genomic locus. However, the …
Efficient high-precision homology-directed repair-dependent genome editing by HDRobust
S Riesenberg, P Kanis, D Macak, D Wollny… - Nature …, 2023 - nature.com
Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …
Methodologies for improving HDR efficiency
M Liu, S Rehman, X Tang, K Gu, Q Fan, D Chen… - Frontiers in …, 2019 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is a precise genome manipulating technology that can be programmed to induce …
(Cas9) is a precise genome manipulating technology that can be programmed to induce …
CRISPR-Cas9: a preclinical and clinical perspective for the treatment of human diseases
At present, the idea of genome modification has revolutionized the modern therapeutic
research era. Genome modification studies have traveled a long way from gene …
research era. Genome modification studies have traveled a long way from gene …
Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans
GA Dokshin, KS Ghanta, KM Piscopo, CC Mello - Genetics, 2018 - academic.oup.com
A robust genome editing pipeline is critical to the vitality of a modern genetic laboratory.
Previous studies have shown that Cas9 ribonucleoprotein (RNP)-based editing can be …
Previous studies have shown that Cas9 ribonucleoprotein (RNP)-based editing can be …
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …
sites. Current off-target discovery methods work using purified DNA or specific cellular …