Isolation, cultivation, and characterization of human mesenchymal stem cells

D Mushahary, A Spittler, C Kasper, V Weber… - Cytometry Part …, 2018 - Wiley Online Library
Mesenchymal stem cells (MSC) exhibit a high self‐renewal capacity, multilineage
differentiation potential and immunomodulatory properties. This set of exceptional features …

Manufacturing of primed mesenchymal stromal cells for therapy

JQ Yin, J Zhu, JA Ankrum - Nature biomedical engineering, 2019 - nature.com
Mesenchymal stromal cells (MSCs) for basic research and clinical applications are
manufactured and developed as unique cell products by many different manufacturers and …

Advancing biomaterials of human origin for tissue engineering

FM Chen, X Liu - Progress in polymer science, 2016 - Elsevier
Biomaterials have played an increasingly prominent role in the success of biomedical
devices and in the development of tissue engineering, which seeks to unlock the …

Human platelet lysate: replacing fetal bovine serum as a gold standard for human cell propagation?

T Burnouf, D Strunk, MBC Koh, K Schallmoser - Biomaterials, 2016 - Elsevier
The essential physiological role of platelets in wound healing and tissue repair builds the
rationale for the use of human platelet derivatives in regenerative medicine. Abundant …

Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy

C Li, X Wu, J Tong, X Yang, J Zhao, Q Zheng… - Stem cell research & …, 2015 - Springer
Abstract Introduction Mesenchymal stem cells (MSCs) are promising candidates for cell-
based therapies. Human platelet lysate represents an efficient alternative to fetal bovine …

The dark side of foetal bovine serum in extracellular vesicle studies

O Urzì, R Olofsson Bagge… - Journal of extracellular …, 2022 - Wiley Online Library
Extracellular vesicles (EVs) have been shown to be involved in cell‐cell communication and
to take part in both physiological and pathological processes. Thanks to their exclusive …

Manufacturing human mesenchymal stem cells at clinical scale: process and regulatory challenges

V Jossen, C van den Bos, R Eibl, D Eibl - Applied microbiology and …, 2018 - Springer
Human mesenchymal stem cell (hMSC)-based therapies are of increasing interest in the
field of regenerative medicine. As economic considerations have shown, allogeneic therapy …

A consensus introduction to serum replacements and serum-free media for cellular therapies

O Karnieli, OM Friedner, JG Allickson, N Zhang, S Jung… - Cytotherapy, 2017 - Elsevier
The cell therapy industry is a fast-growing industry targeted toward a myriad of clinical
indications. As the cell therapy industry matures and clinical trials hit their pivotal Phase 3 …

Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review

M Guiotto, W Raffoul, AM Hart, MO Riehle… - Journal of translational …, 2020 - Springer
Background Foetal bovine serum (FBS), is the most commonly used culture medium additive
for in vitro cultures, despite its undefined composition, its potential immunogenicity and …

Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future

G Astori, E Amati, F Bambi, M Bernardi… - Stem cell research & …, 2016 - Springer
The use of fetal bovine serum (FBS) as a cell culture supplement is discouraged by
regulatory authorities to limit the risk of zoonoses and xenogeneic immune reactions in the …