Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

SA Langhans - Frontiers in pharmacology, 2018 - frontiersin.org
Drug development is a lengthy and costly process that proceeds through several stages
from target identification to lead discovery and optimization, preclinical validation and …

Screening out irrelevant cell-based models of disease

P Horvath, N Aulner, M Bickle, AM Davies… - Nature reviews Drug …, 2016 - nature.com
The common and persistent failures to translate promising preclinical drug candidates into
clinical success highlight the limited effectiveness of disease models currently used in drug …

[HTML][HTML] In Vitro three-dimensional (3D) cell culture tools for spheroid and organoid models

SY Lee, IS Koo, HJ Hwang, DW Lee - SLAS Discovery, 2023 - Elsevier
Abstract Three-dimensional (3D) cell culture technology has been steadily studied since the
1990's due to its superior biocompatibility compared to the conventional two-dimensional …

Biomaterials for three-dimensional cell culture: From applications in oncology to nanotechnology

T Saydé, O El Hamoui, B Alies, K Gaudin, G Lespes… - Nanomaterials, 2021 - mdpi.com
Three-dimensional cell culture has revolutionized cellular biology research and opened the
door to novel discoveries in terms of cellular behavior and response to microenvironment …

Concerns, challenges and promises of high-content analysis of 3D cellular models

N Carragher, F Piccinini, A Tesei, OJT Jr… - Nature reviews Drug …, 2018 - nature.com
© 2018 Mac millan Publishers Li mited, part of Springer Nature. All rights reserved. deeper
penetration of light into tissue, but many areas require improvement, especially if 3D …

Extracellular vesicles from organoids and 3D culture systems

S Abdollahi - Biotechnology and bioengineering, 2021 - Wiley Online Library
When discovered, extracellular vesicles (EVs) such as exosomes were thought of as junk
carriers and a means by which the cell disposed of its waste material. Over the years, the …

High throughput scaffold-based 3D micro-tumor array for efficient drug screening and chemosensitivity testing

X Yan, L Zhou, Z Wu, X Wang, X Chen, F Yang, Y Guo… - Biomaterials, 2019 - Elsevier
Oncology drug development is greatly hampered by inefficient drug screening using 2D
culture. Herein, we present ready-to-use micro-scaffolds in 384-well format to generate …

Using 3D in vitro cell culture models in anti-cancer drug discovery

SA Langhans - Expert Opinion on Drug Discovery, 2021 - Taylor & Francis
Introduction The high failure rate in drug discovery remains a costly and time-consuming
challenge. Improving the odds of success in the early steps of drug development requires …

A 96-well microplate bioreactor platform supporting individual dual perfusion and high-throughput assessment of simple or biofabricated 3D tissue models

J Parrish, KS Lim, K Baer, GJ Hooper, TBF Woodfield - Lab on a Chip, 2018 - pubs.rsc.org
Traditional 2D monolayer cell cultures and submillimeter 3D tissue construct cultures used
widely in tissue engineering are limited in their ability to extrapolate experimental data to …

Physical and biological advances in endothelial cell-based engineered co-culture model systems

CT Mierke - Seminars in Cell & Developmental Biology, 2023 - Elsevier
Scientific knowledge in the field of cell biology and mechanobiology heavily leans on cell-
based in vitro experiments and models that favor the examination and comprehension of …