Best practices for single-cell analysis across modalities

L Heumos, AC Schaar, C Lance, A Litinetskaya… - Nature Reviews …, 2023 - nature.com
Recent advances in single-cell technologies have enabled high-throughput molecular
profiling of cells across modalities and locations. Single-cell transcriptomics data can now …

A reference cell tree will serve science better than a reference cell atlas

S Domcke, J Shendure - Cell, 2023 - cell.com
Single-cell biology is facing a crisis of sorts. Vast numbers of single-cell molecular profiles
are being generated, clustered and annotated. However, this is overwhelmingly ad hoc, and …

scBERT as a large-scale pretrained deep language model for cell type annotation of single-cell RNA-seq data

F Yang, W Wang, F Wang, Y Fang, D Tang… - Nature Machine …, 2022 - nature.com
Annotating cell types on the basis of single-cell RNA-seq data is a prerequisite for research
on disease progress and tumour microenvironments. Here we show that existing annotation …

Advances in spatial transcriptomic data analysis

R Dries, J Chen, N Del Rossi, MM Khan… - Genome …, 2021 - genome.cshlp.org
Spatial transcriptomics is a rapidly growing field that promises to comprehensively
characterize tissue organization and architecture at the single-cell or subcellular resolution …

Consensus prediction of cell type labels in single-cell data with popV

C Ergen, G **ng, C Xu, M Kim, M Jayasuriya… - Nature Genetics, 2024 - nature.com
Cell-type classification is a crucial step in single-cell sequencing analysis. Various methods
have been proposed for transferring a cell-type label from an annotated reference atlas to …

Single-cell transcriptomics for the assessment of cardiac disease

AMA Miranda, V Janbandhu, H Maatz… - Nature Reviews …, 2023 - nature.com
Cardiovascular disease is the leading cause of death globally. An advanced understanding
of cardiovascular disease mechanisms is required to improve therapeutic strategies and …

Spatial omics: Navigating to the golden era of cancer research

Y Wu, Y Cheng, X Wang, J Fan… - Clinical and Translational …, 2022 - Wiley Online Library
The idea that tumour microenvironment (TME) is organised in a spatial manner will not
surprise many cancer biologists; however, systematically capturing spatial architecture of …

Biologically informed deep learning to query gene programs in single-cell atlases

M Lotfollahi, S Rybakov, K Hrovatin… - Nature Cell …, 2023 - nature.com
The increasing availability of large-scale single-cell atlases has enabled the detailed
description of cell states. In parallel, advances in deep learning allow rapid analysis of newly …

Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications

M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …

scDeepSort: a pre-trained cell-type annotation method for single-cell transcriptomics using deep learning with a weighted graph neural network

X Shao, H Yang, X Zhuang, J Liao, P Yang… - Nucleic acids …, 2021 - academic.oup.com
Advances in single-cell RNA sequencing (scRNA-seq) have furthered the simultaneous
classification of thousands of cells in a single assay based on transcriptome profiling. In …