Circularly permuted fluorescent protein-based indicators: history, principles, and classification
Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for
studying the various biological processes in living systems. The circular permutation of …
studying the various biological processes in living systems. The circular permutation of …
Protein kinase C pharmacology: refining the toolbox
PKC (protein kinase C) has been in the limelight since the discovery three decades ago that
it acts as a major receptor for the tumour-promoting phorbol esters. Phorbol esters, with their …
it acts as a major receptor for the tumour-promoting phorbol esters. Phorbol esters, with their …
Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities
Unravelling the dynamic molecular interplay behind complex physiological processes such
as neuronal plasticity requires the ability to both detect minute changes in biochemical …
as neuronal plasticity requires the ability to both detect minute changes in biochemical …
GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation
Strategies aimed at mimicking or enhancing the action of the incretin hormone glucagon-like
peptide 1 (GLP-1) therapeutically improve glucose-stimulated insulin secretion (GSIS); …
peptide 1 (GLP-1) therapeutically improve glucose-stimulated insulin secretion (GSIS); …
Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence
The goal of this work is to determine how GCaMP6m's fluorescence is altered in response to
Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how …
Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how …
Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2
The tip link protein protocadherin 15 (PCDH15) is a central component of the
mechanotransduction complex in auditory and vestibular hair cells. PCDH15 is …
mechanotransduction complex in auditory and vestibular hair cells. PCDH15 is …
Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex
Morphogens function in concentration-dependent manners to instruct cell fate during tissue
patterning. The cytoneme morphogen transport model posits that specialized filopodia …
patterning. The cytoneme morphogen transport model posits that specialized filopodia …
New DAG and cAMP sensors optimized for live-cell assays in automated laboratories
Protein-based, fluorescent biosensors power basic research on cell signaling in health and
disease, but their use in automated laboratories is limited. We have now created two live-cell …
disease, but their use in automated laboratories is limited. We have now created two live-cell …
Interpretation of presynaptic phenotypes of synaptic plasticity in terms of a two-step priming process
E Neher - Journal of General Physiology, 2023 - rupress.org
Studies on synaptic proteins involved in neurotransmitter release often aim at distinguishing
between their roles in vesicle priming (the docking of synaptic vesicles to the plasma …
between their roles in vesicle priming (the docking of synaptic vesicles to the plasma …
Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging
Circuit map** requires knowledge of both structural and functional connectivity between
cells. Although optical tools have been made to assess either the morphology and …
cells. Although optical tools have been made to assess either the morphology and …