X-ray free-electron lasers for the structure and dynamics of macromolecules

HN Chapman - Annual review of biochemistry, 2019 - annualreviews.org
X-ray free-electron lasers provide femtosecond-duration pulses of hard X-rays with a peak
brightness approximately one billion times greater than is available at synchrotron radiation …

[HTML][HTML] From femtoseconds to hours—measuring dynamics over 18 orders of magnitude with coherent x-rays

F Lehmkühler, W Roseker, G Grübel - Applied Sciences, 2021 - mdpi.com
X-ray photon correlation spectroscopy (XPCS) enables the study of sample dynamics
between micrometer and atomic length scales. As a coherent scattering technique, it …

Light-induced structural changes and the site of O= O bond formation in PSII caught by XFEL

M Suga, F Akita, M Sugahara, M Kubo, Y Nakajima… - Nature, 2017 - nature.com
Photosystem II (PSII) is a huge membrane-protein complex consisting of 20 different
subunits with a total molecular mass of 350 kDa for a monomer. It catalyses light-driven …

Extreme focusing of hard X-ray free-electron laser pulses enables 7 nm focus width and 1022 W cm2 intensity

J Yamada, S Matsuyama, I Inoue, T Osaka, T Inoue… - Nature …, 2024 - nature.com
By illuminating matter with bright and intense light, researchers gain insights into material
composition and properties. In the regime of extremely short wavelengths, X-ray free …

Chemical crystallography by serial femtosecond X-ray diffraction

EA Schriber, DW Paley, R Bolotovsky, DJ Rosenberg… - Nature, 2022 - nature.com
Inorganic–organic hybrid materials represent a large share of newly reported structures,
owing to their simple synthetic routes and customizable properties. This proliferation has led …

A three-dimensional movie of structural changes in bacteriorhodopsin

E Nango, A Royant, M Kubo, T Nakane, C Wickstrand… - Science, 2016 - science.org
Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport
protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron …

Visualizing the DNA repair process by a photolyase at atomic resolution

M Maestre-Reyna, PH Wang, E Nango, Y Hosokawa… - Science, 2023 - science.org
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions.
In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a …

Grease matrix as a versatile carrier of proteins for serial crystallography

M Sugahara, E Mizohata, E Nango, M Suzuki… - Nature …, 2015 - nature.com
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution
structural investigation by expanding applicability to micrometer-sized protein crystals, even …

Atomic inner-shell laser at 1.5-ångström wavelength pumped by an X-ray free-electron laser

H Yoneda, Y Inubushi, K Nagamine, Y Michine… - Nature, 2015 - nature.com
Since the invention of the first lasers in the visible-light region, research has aimed to
produce short-wavelength lasers that generate coherent X-rays,; the shorter the wavelength …

Serial femtosecond crystallography reveals that photoactivation in a fluorescent protein proceeds via the hula twist mechanism

A Fadini, CDM Hutchison, D Morozov… - Journal of the …, 2023 - ACS Publications
Chromophore cis/trans photoisomerization is a fundamental process in chemistry and in the
activation of many photosensitive proteins. A major task is understanding the effect of the …