CRISPR-based diagnostics
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
Point-of-care diagnostics for infectious diseases: From methods to devices
C Wang, M Liu, Z Wang, S Li, Y Deng, N He - Nano Today, 2021 - Elsevier
The current widespread of COVID-19 all over the world, which is caused by SARS-CoV-2
virus, has again emphasized the importance of development of point-of-care (POC) …
virus, has again emphasized the importance of development of point-of-care (POC) …
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
CRISPR-Cas12a-based nucleic acid amplification-free DNA biosensor via Au nanoparticle-assisted metal-enhanced fluorescence and colorimetric analysis
Cell-free DNA (cfDNA) has attracted significant attention due to its high potential to diagnose
diseases, such as cancer. Still, its detection by amplification method has limitations because …
diseases, such as cancer. Still, its detection by amplification method has limitations because …
A highly sensitive CRISPR‐empowered surface plasmon resonance sensor for diagnosis of inherited diseases with femtomolar‐level real‐time quantification
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system
has emerged as a promising technology for the detection of nucleic acids. Herein, the …
has emerged as a promising technology for the detection of nucleic acids. Herein, the …
PCR past, present and future
PCR has become one of the most valuable techniques currently used in bioscience,
diagnostics and forensic science. Here we review the history of PCR development and the …
diagnostics and forensic science. Here we review the history of PCR development and the …
Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities
JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
Recent progress in non-native nucleic acid modifications
While Nature harnesses RNA and DNA to store, read and write genetic information, the
inherent programmability, synthetic accessibility and wide functionality of these nucleic acids …
inherent programmability, synthetic accessibility and wide functionality of these nucleic acids …
Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an example
Achieving superhigh sensitivity is the ultimate goal for bio-detection in modern analytical
science and life science. Among variable signal amplification strategies, nucleic acid …
science and life science. Among variable signal amplification strategies, nucleic acid …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …