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[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …
Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …
stability and solvent resistance. However, these modifications are generally associated with …
Importance of the support properties for immobilization or purification of enzymes
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …
Both purification and immobilization have a common factor: they use a solid activated …
Agarose and its derivatives as supports for enzyme immobilization
Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure
that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically …
that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically …
Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties
A heterofunctional support for enzyme immobilization may be defined as that which
possesses several distinct functionalities on its surface able to interact with a protein. We will …
possesses several distinct functionalities on its surface able to interact with a protein. We will …
Advances in enzyme immobilisation
Improvements in current strategies for carrier-based immobilisation have been developed
using hetero-functionalised supports that enhance the binding efficacy and stability through …
using hetero-functionalised supports that enhance the binding efficacy and stability through …
Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance
Mutagenesis and immobilization are usually considered to be unrelated techniques with
potential applications to improve protein properties. However, there are several reports …
potential applications to improve protein properties. However, there are several reports …
Coupling chemical modification and immobilization to improve the catalytic performance of enzymes
Chemical modification and immobilization of enzymes have been usually considered
unrelated tools to improve biocatalyst features. However, there are many examples where a …
unrelated tools to improve biocatalyst features. However, there are many examples where a …
Stability of biocatalysts
Despite their many favorable qualities, the marginal stability of biocatalysts in many types of
reaction media often has prevented or delayed their implementation for industrial-scale …
reaction media often has prevented or delayed their implementation for industrial-scale …
Immobilization of enzymes on heterofunctional epoxy supports
Immobilization of enzymes and proteins on activated supports permits the simplification of
the reactor design and may be used to improve some enzyme properties. In this sense …
the reactor design and may be used to improve some enzyme properties. In this sense …