Carbohydrate-active enzymes (CAZymes) in the gut microbiome
The 1013–1014 microorganisms present in the human gut (collectively known as the human
gut microbiota) dedicate substantial percentages of their genomes to the degradation and …
gut microbiota) dedicate substantial percentages of their genomes to the degradation and …
Emerging roles of O-glycosylation in regulating protein aggregation, phase separation, and functions
Protein O-glycosylation is widely identified in various proteins involved in diverse biological
processes. Recent studies have demonstrated that O-glycosylation plays crucial and …
processes. Recent studies have demonstrated that O-glycosylation plays crucial and …
An engineered bacteria-hybrid microrobot with the magnetothermal bioswitch for remotely collective perception and imaging-guided cancer treatment
H Chen, Y Li, Y Wang, P Ning, Y Shen, X Wei, Q Feng… - ACS …, 2022 - ACS Publications
Microrobots driven by multiple propelling forces hold great potential for noninvasively
targeted delivery in the physiologic environment. However, the remotely collective …
targeted delivery in the physiologic environment. However, the remotely collective …
A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
Mucin-type O-glycosylation is a post-translational modification present at the interface
between cells where it has important roles in cellular communication. However, deciphering …
between cells where it has important roles in cellular communication. However, deciphering …
An orthogonal metabolic framework for one-carbon utilization
Metabolic engineering often entails concurrent engineering of substrate utilization, central
metabolism and product synthesis pathways, inevitably creating interdependency with …
metabolism and product synthesis pathways, inevitably creating interdependency with …
Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane …
attention for biomanufacturing and synthetic biology. However, activating membrane …
Bacterial glycoengineering: cell-based and cell-free routes for producing biopharmaceuticals with customized glycosylation
Glycosylation plays a pivotal role in tuning the folding and function of proteins. Because
most human therapeutic proteins are glycosylated, understanding and controlling …
most human therapeutic proteins are glycosylated, understanding and controlling …
O-glycosylation and its role in therapeutic proteins
N Thompson, W Wakarchuk - Bioscience Reports, 2022 - portlandpress.com
Protein glycosylation is ubiquitous throughout biology. From bacteria to humans, this post
translational modification with sophisticated carbohydrate structures plays a profound role in …
translational modification with sophisticated carbohydrate structures plays a profound role in …
Cell-free systems for accelerating glycoprotein expression and biomanufacturing
Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar
moieties, plays critical roles in cellular function, human health, and biotechnology. However …
moieties, plays critical roles in cellular function, human health, and biotechnology. However …