Two-photon calcium imaging of neuronal activity

C Grienberger, A Giovannucci, W Zeiger… - Nature Reviews …, 2022 - nature.com
In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal
activity in the intact brain. It is based on the principle that, when neurons fire action …

Probing neural codes with two-photon holographic optogenetics

H Adesnik, L Abdeladim - Nature neuroscience, 2021 - nature.com
Optogenetics ushered in a revolution in how neuroscientists interrogate brain function.
Because of technical limitations, the majority of optogenetic studies have used low spatial …

[HTML][HTML] Fast and sensitive GCaMP calcium indicators for imaging neural populations

Y Zhang, M Rózsa, Y Liang, D Bushey, Z Wei, J Zheng… - Nature, 2023 - nature.com
Calcium imaging with protein-based indicators, is widely used to follow neural activity in
intact nervous systems, but current protein sensors report neural activity at timescales much …

High-performance calcium sensors for imaging activity in neuronal populations and microcompartments

H Dana, Y Sun, B Mohar, BK Hulse, AM Kerlin… - Nature …, 2019 - nature.com
Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to
measure neural activity in intact nervous systems. GECIs are frequently used in one of two …

Spontaneous behaviors drive multidimensional, brainwide activity

C Stringer, M Pachitariu, N Steinmetz, CB Reddy… - Science, 2019 - science.org
INTRODUCTION In the absence of sensory inputs, the brain produces structured patterns of
activity, which can be as large as or larger than sensory-driven activity. Ongoing activity …

Reprogramming tumour-associated macrophages to outcompete cancer cells

X Zhang, S Li, I Malik, MH Do, L Ji, C Chou, W Shi… - Nature, 2023 - nature.com
In metazoan organisms, cell competition acts as a quality control mechanism to eliminate
unfit cells in favour of their more robust neighbours,. This mechanism has the potential to be …

Suite2p: beyond 10,000 neurons with standard two-photon microscopy

M Pachitariu, C Stringer, S Schröder, M Dipoppa… - BioRxiv, 2016 - biorxiv.org
The combination of two-photon microscopy recordings and powerful calcium-dependent
fluorescent sensors enables simultaneous recording of unprecedentedly large populations …

Map** of brain activity by automated volume analysis of immediate early genes

N Renier, EL Adams, C Kirst, Z Wu, R Azevedo, J Kohl… - Cell, 2016 - cell.com
Understanding how neural information is processed in physiological and pathological states
would benefit from precise detection, localization, and quantification of the activity of all …

Sensitive red protein calcium indicators for imaging neural activity

H Dana, B Mohar, Y Sun, S Narayan, A Gordus… - elife, 2016 - elifesciences.org
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large
populations of neurons and in small neuronal compartments, over times of milliseconds to …

Genetic dissection of neural circuits: a decade of progress

L Luo, EM Callaway, K Svoboda - Neuron, 2018 - cell.com
Tremendous progress has been made since Neuron published our Primer on genetic
dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical …