Modified nucleic acids: replication, evolution, and next-generation therapeutics

K Duffy, S Arangundy-Franklin, P Holliger - Bmc Biology, 2020 - Springer
Modified nucleic acids, also called xeno nucleic acids (XNAs), offer a variety of advantages
for biotechnological applications and address some of the limitations of first-generation …

Detecting RNA and DNA with templated chemical reactions

AP Silverman, ET Kool - Chemical reviews, 2006 - ACS Publications
Sensing nucleic acid sequences is critical in modern-day biology and medicine, and is likely
to continue to increase in importance over the next 10r20 years. The current uses for …

Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly

J Jacobson, LLY Chu, S Ramu - US Patent 10,207,240, 2019 - Google Patents
US10207240B2 - Methods and microfluidic devices for the manipulation of droplets in high
fidelity polynucleotide assembly - Google Patents US10207240B2 - Methods and microfluidic …

Ligation assembly and detection of polynucleotides on solid-support

MW Hunkapiller, AC Hiatt - US Patent 5,942,609, 1999 - Google Patents
BACKGROUND The manipulation of functional gene sequences is the basis of molecular
cloning. Ready availability of synthetic genes at a reasonable cost Will accelerate the …

Methods and devices for high fidelity polynucleotide synthesis

J Jacobson, G Church, LLY Chu - US Patent 8,808,986, 2014 - Google Patents
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide
product having a prede? ned sequence from a plurality of different oligonucleotides. In …

Methods for sorting nucleic acids and multiplexed preparative in vitro cloning

J Jacobson, MJ Goldberg, AK Li-yun… - US Patent …, 2018 - Google Patents
Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids
using high throughput sequencing. Specifically, nucleic acid molecules having the desired …

Detection of nucleic acid targets using chemically reactive oligonucleotide probes

R Terbrueggen - US Patent 10,829,803, 2020 - Google Patents
The present invention provides compositions, apparatuses and methods for detecting one or
more nucleic acid targets present in a sample. Methods of the invention include utilizing two …

Methods and compositions for detecting target nucleic acids

R Terbrueggen, Y Liu, JR Childers, CH Kim… - US Patent …, 2018 - Google Patents
The present invention provides compositions, apparatuses and methods for detecting one or
more nucleic acid targets present in a sample. Methods of the invention include utilizing two …

Methods and devices for high fidelity polynucleotide synthesis

J Jacobson, G Church, LLY Chu - US Patent 9,856,471, 2018 - Google Patents
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide
product having a predefined sequence from a plurality of different oligonucleotides. In …

Protein arrays and methods of using and making the same

J Jacobson, ME Hudson, LLY Chu - US Patent 10,457,935, 2019 - Google Patents
Methods and devices are provided for preparing a protein array having a plurality of
proteins. In one embodiment, the method includes providing a plurality of nucleic acids each …