Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Imaging large fields of view while preserving high-resolution structural information remains a
challenge in low-dose cryo-electron tomography. Here we present robust tools for montage …

In this study, we used cryoelectron microscopy to determine the structures of the Flotillin
protein complex, part of the Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) superfamily …

Extracellular vesicles (EVs) are cell‐released, heterogenous nanoparticles that play
important roles in (patho) physiological processes through intercellular communication. EVs …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Cryo-electron tomography (cryo-ET) has become a powerful approach to study the high-
resolution structure of cellular macromolecular machines in situ. However, the current …

Extracellular vesicles (EVs) are small membrane-bound vesicles released by cells. EVs are
emerging as a promising class of therapeutic entity that could be adapted in formulation due …

The neuronal axon is packed with cytoskeletal filaments, membranes, and organelles, many
of which move between the cell body and axon tip. Here, we used cryo-electron tomography …

Ebola viruses (EBOVs) assemble into filamentous virions, whose shape and stability are
determined by the matrix viral protein 40 (VP40). Virus entry into host cells occurs via …