Recent progress in engineering highly promising biocatalysts has increasingly involved
machine learning methods. These methods leverage existing experimental and simulation …

This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Enzyme design faces challenges related to the implementation of the basic principles that
govern the catalytic activity in natural enzymes. In this work, we revisit basic electrostatic …

Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic
acid and α-L-guluronic acid linked by α, β-(1, 4)-glycosidic bonds. Alginate lyases degrade …

Enzymes offer a more environmentally friendly and low-impact solution to conventional
chemistry, but they often require additional engineering for their application in industrial …

Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes
frequently lack the requisite attributes for effective organic synthesis. The inherent limitations …

Leucine dehydrogenase (LeuDH, EC 1.4. 1.9) can reversibly catalyze the oxidative
deamination of l-leucine and some other specific α-amino acids to form the corresponding α …

In the field of food processing, enzymes play a pivotal role in improving product quality and
flavor, and extending shelf life. However, the exposure of traditional food enzymes to high …

When it comes to enzyme stability and their application in organic solvents, enzyme
biocatalysis has emerged as a popular substitute for conventional chemical processes …

The improvement of enzyme thermostability often accompanies the decreased activity due to
the loss of the key regions' flexibility. As a representative structure, unlocking the potential of …