Reduced nicotinamide adenine nucleotide phosphate (NADPH), which is one of the key
cofactors in the metabolic network, plays an important role in the biochemical reactions, and …

Amino acid dehydrogenases (AADHs) are a group of enzymes that catalyze the reversible
reductive amination of keto acids with ammonia to produce chiral amino acids using either …

Glutamate dehydrogenase (GDH) releases ammonia in a reversible NAD (P)+-dependent
oxidative deamination of glutamate that yields 2-oxoglutarate (2OG). In current perception …

Biosynthesizing unnatural chiral amino acids is challenging due to the limited reductive
amination activity of amino acid dehydrogenase (AADH). Here, for the asymmetric synthesis …

The objective of this study was to identify and exploit a robust biocatalyst that can be applied
in reductive amination for enantioselective synthesis of the competitive herbicide L …

Kinetic aspects of enzymatic reactions are described by equations based on the Michaelis–
Menten theory for the initial stage. However, the kinetic parameters provide little information …

Background Corynebacterium glutamicum is a non-pathogenic bacterium widely used in
industrial amino acid production and metabolic engineering research. Although the genome …

Glutamate dehydrogenase (GDH) is a key enzyme connecting carbon and nitrogen
metabolism in all living organisms. Despite extensive studies on GDHs from both prokaryotic …

Glutamate dehydrogenases (GluDHs) are promising biocatalysts for the synthesis of chiral α‐
amino acids by asymmetric reductive amination of α‐keto acids. However, their strict …

In this study, a novel enzymatic approach to transform levulinic acid (LA), which can be
obtained from biomass, into value-added (R)-4-aminopentanoic acid using an engineered …