Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …

Computational proteomics is the data science concerned with the identification and
quantification of proteins from high-throughput data and the biological interpretation of their …

The dia-PASEF technology uses ion mobility separation to reduce signal interferences and
increase sensitivity in proteomic experiments. Here we present a two-dimensional peak …

Metabolic efficiency profoundly influences organismal fitness. Nonphotosynthetic organisms,
from yeast to mammals, derive usable energy primarily through glycolysis and respiration …

Mass spectrometry is unmatched in its versatility for studying practically any aspect of the
proteome. Because the foundations of mass spectrometry-based proteomics are complex …

Clear cell renal cell carcinomas (ccRCCs) represent∼ 75% of RCC cases and account for
most RCC-associated deaths. Inter-and intratumoral heterogeneity (ITH) results in varying …

Background Macrophages are innate immune cells with diverse functional and molecular
phenotypes. This diversity is largely unexplored at the level of single-cell proteomes …

Defining the cellular response to pharmacological agents is critical for understanding the
mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate …

The development of the TMTpro-16plex series expanded the breadth of commercial isobaric
tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described …

Isobaric labeling empowers proteome-wide expression measurements simultaneously
across multiple samples. Here an expanded set of 16 isobaric reagents based on an …