Overcoming the pitfalls of merging dietary metabarcoding into ecological networks

JP Cuff, FM Windsor, MPTG Tercel… - Methods in Ecology …, 2022 - Wiley Online Library
The construction of increasingly detailed species interaction networks is extending the
potential applications of network ecology, providing an opportunity to understand complex …

[HTML][HTML] Key factors to consider in the use of environmental DNA metabarcoding to monitor terrestrial ecological restoration

M van der Heyde, M Bunce, P Nevill - Science of the Total Environment, 2022 - Elsevier
Ecological restoration of terrestrial environments is a globally important process to combat
the loss of biodiversity and ecosystem services. Holistic monitoring of restored biota and …

Validation of COI metabarcoding primers for terrestrial arthropods

V Elbrecht, TWA Braukmann, NV Ivanova, SWJ Prosser… - PeerJ, 2019 - peerj.com
Metabarcoding can rapidly determine the species composition of bulk samples and thus
aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that …

Monitoring the birds and the bees: Environmental DNA metabarcoding of flowers detects plant–animal interactions

JP Newton, PW Bateman, MJ Heydenrych… - Environmental …, 2023 - Wiley Online Library
Animal pollinators are vital for the reproduction of~ 90% of flowering plants. However, many
of these pollinating species are experiencing declines globally, making effective pollinator …

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

F Leese, M Sander, D Buchner, V Elbrecht… - Environmental …, 2021 - Wiley Online Library
DNA metabarcoding of freshwater communities typically relies on PCR amplification of a
fragment of the mitochondrial cytochrome c oxidase I (COI) gene with degenerate primers …

[HTML][HTML] TICI: a taxon-independent community index for eDNA-based ecological health assessment

SP Wilkinson, AA Gault, SA Welsh, JP Smith, BO David… - PeerJ, 2024 - peerj.com
Global biodiversity is declining at an ever-increasing rate. Yet effective policies to mitigate or
reverse these declines require ecosystem condition data that are rarely available …

Why we need sustainable networks bridging countries, disciplines, cultures and generations for aquatic biomonitoring 2.0: a perspective derived from the DNAqua-Net …

F Leese, A Bouchez, K Abarenkov, F Altermatt… - Advances in ecological …, 2018 - Elsevier
Aquatic biomonitoring has become an essential task in Europe and many other regions as a
consequence of strong anthropogenic pressures affecting the health of lakes, rivers, oceans …

Advancing the integration of multi‐marker metabarcoding data in dietary analysis of trophic generalists

LP Da Silva, VA Mata, PB Lopes… - Molecular Ecology …, 2019 - Wiley Online Library
The application of DNA metabarcoding to dietary analysis of trophic generalists requires
using multiple markers in order to overcome problems of primer specificity and bias …

Assessing the response of an urban stream ecosystem to salinization under different flow regimes

IM Pimentel, D Baikova, D Buchner… - Science of The Total …, 2024 - Elsevier
Urban streams are exposed to a variety of anthropogenic stressors. Freshwater salinization
is a key stressor in these ecosystems that is predicted to be further exacerbated by climate …

Estimating intraspecific genetic diversity from community DNA metabarcoding data

V Elbrecht, EE Vamos, D Steinke, F Leese - PeerJ, 2018 - peerj.com
Background DNA metabarcoding is used to generate species composition data for entire
communities. However, sequencing errors in high-throughput sequencing instruments are …