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Identifying biases and their potential solutions in human microbiome studies
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …
Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …
Deep learning and computer vision will transform entomology
Most animal species on Earth are insects, and recent reports suggest that their abundance is
in drastic decline. Although these reports come from a wide range of insect taxa and regions …
in drastic decline. Although these reports come from a wide range of insect taxa and regions …
Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data?
Advances in DNA sequencing technology have revolutionized the field of molecular analysis
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …
An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar
How viruses evolve within hosts can dictate infection outcomes; however, reconstructing this
process is challenging. We evaluate our multiplexed amplicon approach, PrimalSeq, to …
process is challenging. We evaluate our multiplexed amplicon approach, PrimalSeq, to …
Consistent and correctable bias in metagenomic sequencing experiments
Marker-gene and metagenomic sequencing have profoundly expanded our ability to
measure biological communities. But the measurements they provide differ from the truth …
measure biological communities. But the measurements they provide differ from the truth …
Toward quantitative metabarcoding
Amplicon‐sequence data from environmental DNA (eDNA) and microbiome studies provide
important information for ecology, conservation, management, and health. At present …
important information for ecology, conservation, management, and health. At present …
Biases in bulk: DNA metabarcoding of marine communities and the methodology involved
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …
Genome-wide analysis of somatic noncoding mutation patterns in cancer
We established a genome-wide compendium of somatic mutation events in 3949 whole
cancer genomes representing 19 tumor types. Protein-coding events captured well …
cancer genomes representing 19 tumor types. Protein-coding events captured well …
Critical insight into recombinase polymerase amplification technology
MA Munawar - Expert Review of Molecular Diagnostics, 2022 - Taylor & Francis
Introduction Recombinase polymerase amplification (RPA) is a promising and emerging
technology for rapidly amplifying target nucleic acid from minimally processed samples and …
technology for rapidly amplifying target nucleic acid from minimally processed samples and …