George Weber was among the first to recognize that extensive metabolic changes must
underlie the unbridled proliferation of cancer cells. 1 His molecular correlation hypothesis …

Antifolate antimalarial drugs interfere with folate metabolism, a pathway essential to malaria
parasite survival. This class of drugs includes effective causal prophylactic and therapeutic …

Directed evolution is a powerful approach for engineering biomolecules and understanding
adaptation. However, experimental strategies for directed evolution are notoriously labor …

The widely used pESC vector series (Stratagene, La Jolla, CA, USA) with the bidirectional
GAL1/GAL10 promoter provides the possibility of simultaneously expressing two different …

Plasmodium falciparum dihydrofolate reductase–thymidylate synthase (PfDHFR-TS) is an
important target of antimalarial drugs. The efficacy of this class of DHFR-inhibitor drugs is …

Analyses of mitochondrial cytochrome b diversity among avian blood parasites of the genera
Haemoproteus and Plasmodium suggest that there might be as many lineages of parasites …

Chemotherapy remains the most important means of controlling malaria, one of the
deadliest infectious parasitic diseases in the world. Antimalarial antifolates have been …

Tetrahydrofolate cofactors are essential for the synthesis of purines, certain amino acids,
and thymidine. Most bacteria and plants produce these folate cofactors by de novo …

The antifolates were the first class of antimetabolites to enter the clinics more than 50 years
ago. Over the following decades, a full understanding of their mechanisms of action and …

Plasmodium falciparum is the causative agent of the most serious and fatal malarial
infections, and it has developed resistance to commonly employed chemotherapeutics. The …