Hydrogen exchange mass spectrometry for the analysis of protein dynamics
Hydrogen exchange coupled to mass spectrometry (MS) has become a valuable analytical
tool for the study of protein dynamics. By combining information about protein dynamics with …
tool for the study of protein dynamics. By combining information about protein dynamics with …
Mechanisms and uses of hydrogen exchange
Recent work has largely completed our understanding of the hydrogen-exchange chemistry
of unstructured proteins and nucleic acids. Some of the high-energy structural fluctuations …
of unstructured proteins and nucleic acids. Some of the high-energy structural fluctuations …
Hydrogen exchange: the modern legacy of Linderstrøm‐Lang
This discussion, prepared for the Protein Society's symposium honoring the 100th
anniversary of Kaj Linderstrøm‐Lang, shows how hydrogen exchange approaches initially …
anniversary of Kaj Linderstrøm‐Lang, shows how hydrogen exchange approaches initially …
Protein analysis by hydrogen exchange mass spectrometry
AN Hoofnagle, KA Resing… - Annual review of …, 2003 - annualreviews.org
▪ Abstract Mass spectrometry has provided a powerful method for monitoring hydrogen
exchange of protein backbone amides with deuterium from solvent. In comparison to …
exchange of protein backbone amides with deuterium from solvent. In comparison to …
Detection of rare partially folded molecules in equilibrium with the native conformation of RNaseH
AK Chamberlain, TM Handel, S Marqusee - Nature structural biology, 1996 - nature.com
Despite the general observation that single domain proteins denature in a completely
cooperative manner, amide hydrogen exchange of ribonuclease H in low levels of …
cooperative manner, amide hydrogen exchange of ribonuclease H in low levels of …
Structure-based calculation of the equilibrium folding pathway of proteins. Correlation with hydrogen exchange protection factors
A new statistical thermodynamic formalism has been developed in order to describe the
equilibrium folding pathway of proteins. The resulting formalism allows calculation of the …
equilibrium folding pathway of proteins. The resulting formalism allows calculation of the …
The structural distribution of cooperative interactions in proteins: analysis of the native state ensemble
Cooperative interactions link the behavior of different amino acid residues within a protein
molecule. As a result, the effects of chemical or physical perturbations to any given residue …
molecule. As a result, the effects of chemical or physical perturbations to any given residue …
A statistical thermodynamic model of the protein ensemble
To understand the structural basis of biology, it is necessary to understand the
transformations of macromolecules during functional cycles. These changes can involve …
transformations of macromolecules during functional cycles. These changes can involve …
Hydrogen exchange in peptides and proteins using NMR spectroscopy
CE Dempsey - Progress in Nuclear Magnetic Resonance …, 2001 - Elsevier
Analysis of the kinetics of hydrogen exchange in biological macromolecules is a powerful
method for studying both their structural and dynamic properties. This arises from our …
method for studying both their structural and dynamic properties. This arises from our …
Structural stability of binding sites: consequences for binding affinity and allosteric effects
During the course of biological function, proteins interact with other proteins, ligands,
substrates, inhibitors, etc. These interactions occur at precisely defined locations within the …
substrates, inhibitors, etc. These interactions occur at precisely defined locations within the …