Harnessing the power of RADseq for ecological and evolutionary genomics
High-throughput techniques based on restriction site-associated DNA sequencing (RADseq)
are enabling the low-cost discovery and genoty** of thousands of genetic markers for any …
are enabling the low-cost discovery and genoty** of thousands of genetic markers for any …
Phylogenomics and the flowering plant tree of life
The advances accelerated by next‐generation sequencing and long‐read sequencing
technologies continue to provide an impetus for plant phylogenetic study. In the past …
technologies continue to provide an impetus for plant phylogenetic study. In the past …
Stacks 2: Analytical methods for paired‐end sequencing improve RADseq‐based population genomics
For half a century population genetics studies have put type II restriction endonucleases to
work. Now, coupled with massively‐parallel, short‐read sequencing, the family of RAD …
work. Now, coupled with massively‐parallel, short‐read sequencing, the family of RAD …
Lost in parameter space: a road map for stacks
Restriction site‐Associated DNA sequencing (RAD‐seq) has become a widely adopted
method for genoty** populations of model and non‐model organisms. Generating a …
method for genoty** populations of model and non‐model organisms. Generating a …
Circulating tumor DNA and liquid biopsy: opportunities, challenges, and recent advances in detection technologies
Cell-free DNA (cfDNA) refers to short fragments of acellular nucleic acids detectable in
almost all body fluids, including blood, and is involved in various physiological and …
almost all body fluids, including blood, and is involved in various physiological and …
These aren't the loci you'e looking for: Principles of effective SNP filtering for molecular ecologists
Sequencing reduced‐representation libraries of restriction site‐associated DNA (RAD seq)
to identify single nucleotide polymorphisms (SNP s) is quickly becoming a standard …
to identify single nucleotide polymorphisms (SNP s) is quickly becoming a standard …
Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext)
Massively parallel DNA sequencing offers many benefits, but major inhibitory cost factors
include:(1) start-up (ie, purchasing initial reagents and equipment);(2) buyin (ie, getting the …
include:(1) start-up (ie, purchasing initial reagents and equipment);(2) buyin (ie, getting the …
Practical considerations for plant phylogenomics
The past decade has seen a major breakthrough in our ability to easily and inexpensively
sequence genome‐scale data from diverse lineages. The development of high‐throughput …
sequence genome‐scale data from diverse lineages. The development of high‐throughput …
Comparing the Performance of Microsatellites and RADseq in Population Genetic Studies: Analysis of Data for Pike (Esox lucius) and a Synthesis of Previous Studies
Population genetic studies reveal biodiversity patterns and inform about drivers of
evolutionary differentiation and adaptation, including gene flow, drift and selection. This can …
evolutionary differentiation and adaptation, including gene flow, drift and selection. This can …
Sequence capture of ultraconserved elements from bird museum specimens
New DNA sequencing technologies are allowing researchers to explore the genomes of the
millions of natural history specimens collected prior to the molecular era. Yet, we know little …
millions of natural history specimens collected prior to the molecular era. Yet, we know little …