Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

G Muyzer, K Smalla - Antonie van Leeuwenhoek, 1998 - Springer
Here, the state of the art of the application of denaturing gradient gel electrophoresis
(DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be …

Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis

F v. Wintzingerode, UB Göbel… - FEMS microbiology …, 1997 - academic.oup.com
After nearly 10 years of PCR-based analysis of prokaryotic small-subunit ribosomal RNAs
for ecological studies it seems necessary to summarize reported pitfalls of this approach …

The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations

JH Rotthauwe, KP Witzel, W Liesack - Applied and environmental …, 1997 - Am Soc Microbiol
The naturally occurring genetic heterogeneity of autotrophic ammonia-oxidizing populations
belonging to the beta subclass of the Proteobacteria was studied by using a newly …

PCR primers to amplify 16S rRNA genes from cyanobacteria

U Nübel, F Garcia-Pichel, G Muyzer - Applied and environmental …, 1997 - Am Soc Microbiol
We developed and tested a set of oligonucleotide primers for the specific amplification of
16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were …

Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding …

L Ovreås, L Forney, FL Daae… - Applied and …, 1997 - Am Soc Microbiol
The community structure of bacterioplankton in meromictic Lake Saelenvannet was
examined by PCR amplification of the V3 region of 16S rRNA from microbial communities …

Effect of pH on hydrogen production from glucose by a mixed culture

HHP Fang, H Liu - Bioresource technology, 2002 - Elsevier
The effect of pH on the conversion of glucose to hydrogen by a mixed culture of fermentative
bacteria was evaluated. At 36° C, six hours hydraulic retention, over 90% of glucose was …

Soil and plant specific effects on bacterial community composition in the rhizosphere

P Marschner, CH Yang, R Lieberei… - Soil biology and …, 2001 - Elsevier
Eubacterial community structures in the plant rhizosphere were examined with respect to
plant species, soil type, and root zone location. Three plant species (chickpea, rape and …

Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation

P Regmi, MW Miller, B Holgate, R Bunce, H Park… - Water research, 2014 - Elsevier
This work describes the development of an intermittently aerated pilot-scale process (V=
0.34 m 3) operated without oxidized nitrogen recycle and supplemental carbon addition …

Microbial evolution, diversity, and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms

IM Head, JR Saunders, RW Pickup - Microbial ecology, 1998 - Springer
A bstract The application of molecular biological methods to study the diversity and ecology
of microorganisms in natural environments has been practiced since the mid-1980s. Since …

PCR-DGGE fingerprinting: novel strategies for detection of microbes in food

D Ercolini - Journal of microbiological methods, 2004 - Elsevier
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE)
fingerprinting was recently introduced into food microbiology. This paper describes the …