Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Super-resolution structured illumination microscopy

R Heintzmann, T Huser - Chemical reviews, 2017 - ACS Publications
Super-resolved structured illumination microscopy (SR-SIM) is among the most rapidly
growing fluorescence microscopy techniques that can surpass the optical diffraction limit …

Once and only once: mechanisms of centriole duplication and their deregulation in disease

EA Nigg, AJ Holland - Nature reviews Molecular cell biology, 2018 - nature.com
Centrioles are conserved microtubule-based organelles that form the core of the centrosome
and act as templates for the formation of cilia and flagella. Centrioles have important roles in …

The centrosome is a selective condensate that nucleates microtubules by concentrating tubulin

JB Woodruff, BF Gomes, PO Widlund, J Mahamid… - Cell, 2017 - cell.com
Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays.
They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic …

Centrosome function and assembly in animal cells

PT Conduit, A Wainman, JW Raff - Nature reviews Molecular cell …, 2015 - nature.com
It has become clear that the role of centrosomes extends well beyond that of important
microtubule organizers. There is increasing evidence that they also function as coordination …

Three-dimensional structured illumination microscopy with enhanced axial resolution

X Li, Y Wu, Y Su, I Rey-Suarez, C Matthaeus… - Nature …, 2023 - nature.com
The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is
limited to∼ 300 nm. Here we present two distinct, complementary methods to improve axial …

Reversible centriole depletion with an inhibitor of Polo-like kinase 4

YL Wong, JV Anzola, RL Davis, M Yoon, A Motamedi… - Science, 2015 - science.org
Centrioles are ancient organelles that build centrosomes, the major microtubule-organizing
centers of animal cells. Extra centrosomes are a common feature of cancer cells. To …

Mechanism and regulation of centriole and cilium biogenesis

DK Breslow, AJ Holland - Annual review of biochemistry, 2019 - annualreviews.org
The centriole is an ancient microtubule-based organelle with a conserved nine-fold
symmetry. Centrioles form the core of centrosomes, which organize the interphase …

Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ

M Müller, V Mönkemöller, S Hennig, W Hübner… - Nature …, 2016 - nature.com
Super-resolved structured illumination microscopy (SR-SIM) is an important tool for
fluorescence microscopy. SR-SIM microscopes perform multiple image acquisitions with …

Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material

S Lawo, M Hasegan, GD Gupta, L Pelletier - Nature cell biology, 2012 - nature.com
The centrosome is the main microtubule organization centre of animal cells. It is composed
of a centriole pair surrounded by pericentriolar material (PCM). Traditionally described as …