Advances in ultrahigh-throughput screening for directed enzyme evolution
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
Droplet based microfluidics
Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining
soft matter physics, biochemistry and microsystems engineering. Its applications range from …
soft matter physics, biochemistry and microsystems engineering. Its applications range from …
Development and future of droplet microfluidics
Over the past two decades, advances in droplet-based microfluidics have facilitated new
approaches to process and analyze samples with unprecedented levels of precision and …
approaches to process and analyze samples with unprecedented levels of precision and …
Surfactants in droplet-based microfluidics
JC Baret - Lab on a Chip, 2012 - pubs.rsc.org
Surfactants are an essential part of the droplet-based microfluidic technology. They are
involved in the stabilization of droplet interfaces, in the biocompatibility of the system and in …
involved in the stabilization of droplet interfaces, in the biocompatibility of the system and in …
Droplet microfluidics—A tool for single‐cell analysis
HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology
Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …
biological research. They provide a compartment in which species or reactions can be …
Revisiting lab-on-a-chip technology for drug discovery
The field of microfluidics or lab-on-a-chip technology aims to improve and extend the
possibilities of bioassays, cell biology and biomedical research based on the idea of …
possibilities of bioassays, cell biology and biomedical research based on the idea of …
Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity
We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS)
combining many of the advantages of microtitre-plate screening and traditional fluorescence …
combining many of the advantages of microtitre-plate screening and traditional fluorescence …
Analytical detection techniques for droplet microfluidics—A review
Y Zhu, Q Fang - Analytica chimica acta, 2013 - Elsevier
In the last decade, droplet-based microfluidics has undergone rapid progress in the fields of
single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has …
single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has …
Accurate microfluidic sorting of droplets at 30 kHz
A Sciambi, AR Abate - Lab on a Chip, 2015 - pubs.rsc.org
Fluorescence-activated droplet sorting is an important tool for droplet microfluidic workflows,
but published approaches are unable to surpass throughputs of a few kilohertz. We present …
but published approaches are unable to surpass throughputs of a few kilohertz. We present …