Advances in ultrahigh-throughput screening for directed enzyme evolution

U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

Droplet based microfluidics

R Seemann, M Brinkmann, T Pfohl… - Reports on progress …, 2011 - iopscience.iop.org
Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining
soft matter physics, biochemistry and microsystems engineering. Its applications range from …

Development and future of droplet microfluidics

L Nan, H Zhang, DA Weitz, HC Shum - Lab on a Chip, 2024 - pubs.rsc.org
Over the past two decades, advances in droplet-based microfluidics have facilitated new
approaches to process and analyze samples with unprecedented levels of precision and …

Surfactants in droplet-based microfluidics

JC Baret - Lab on a Chip, 2012 - pubs.rsc.org
Surfactants are an essential part of the droplet-based microfluidic technology. They are
involved in the stabilization of droplet interfaces, in the biocompatibility of the system and in …

Droplet microfluidics—A tool for single‐cell analysis

HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …

Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

AB Theberge, F Courtois, Y Schaerli… - Angewandte Chemie …, 2010 - Wiley Online Library
Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …

Revisiting lab-on-a-chip technology for drug discovery

P Neužil, S Giselbrecht, K Länge, TJ Huang… - Nature reviews Drug …, 2012 - nature.com
The field of microfluidics or lab-on-a-chip technology aims to improve and extend the
possibilities of bioassays, cell biology and biomedical research based on the idea of …

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

JC Baret, OJ Miller, V Taly, M Ryckelynck, A El-Harrak… - Lab on a Chip, 2009 - pubs.rsc.org
We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS)
combining many of the advantages of microtitre-plate screening and traditional fluorescence …

Analytical detection techniques for droplet microfluidics—A review

Y Zhu, Q Fang - Analytica chimica acta, 2013 - Elsevier
In the last decade, droplet-based microfluidics has undergone rapid progress in the fields of
single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has …

Accurate microfluidic sorting of droplets at 30 kHz

A Sciambi, AR Abate - Lab on a Chip, 2015 - pubs.rsc.org
Fluorescence-activated droplet sorting is an important tool for droplet microfluidic workflows,
but published approaches are unable to surpass throughputs of a few kilohertz. We present …