The task of transcribing nuclear genes is shared between three RNA polymerases in
eukaryotes: RNA polymerase (pol) I synthesises the large rRNA, pol II synthesises mRNA …

RNA polymerase (Pol) III transcribes small untranslated RNAs such as 5S ribosomal RNA,
transfer RNAs, and U6 small nuclear RNA. Because of the functions of these RNAs, Pol III …

The highly abundant, small stable RNAs that are synthesized by RNA polymerase III
(RNAPIII) have key functional roles, particularly in the protein synthesis apparatus. Their …

The chromosomes of eukaryotes are organized into structurally and functionally discrete
domains. Several DNA elements have been identified that act to separate these chromatin …

Transcription complexes that assemble on tRNA genes in a crude Saccharomyces
cerevisiae cell extract extend over the entire transcription unit and approximately 40 base …

Regulation of eukaryotic gene expression involves a dynamic interplay between the core
transcriptional machinery, transcription factors, and chromatin organization and modification …

Abstract In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for transcribing
genes encoding tRNAs and other short non-coding RNAs. The recruitment of Pol III to tRNA …

We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed
genes in ten yeast genomes. This set includes all tRNA genes (tDNA) and genes coding for …

Transcription of transfer RNA (tRNA) genes by RNA polymerase (Pol) III requires the general
transcription factor IIIC (TFIIIC), which recognizes intragenic A-box and B-box DNA motifs of …

We present a new method for rapid purification to near homogeneity of sequence specific
DNA binding proteins based on magnetic separation. The method is described for the …