Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural
protein sources rarely meet the requirements for quantity, ease of isolation or price and …

Protein expression in Escherichia coli represents the most facile approach for the
preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the …

We introduce a method for sequence homology–independent protein recombination
(SHIPREC) that can create libraries of single-crossover hybrids of unrelated or distantly …

The primary component of amyloid plaque in the brains of Alzheimer's patients is the 42
residue amyloid-β-peptide (Aβ42). Although the amino acid residue sequence of Aβ42 is …

By providing a simple and reliable route to enzyme improvement, directed evolution has
emerged as a key technology for enzyme engineering and biocatalysis. Recent advances …

The availability of vast gene repertoires from both natural sources (genomic and cDNA
libraries) and artificial sources (gene libraries) demands the development and application of …

Drug‐induced proteome stress that involves protein aggregation may cause adverse effects
and undermine the safety profile of a drug. Safety of drugs is regularly evaluated using …

We have developed, experimentally implemented, and modeled in silico a methodology
named SCRATCHY that enables the combinatorial engineering of target proteins …

Protein misfolding is the basis of a number of human diseases and presents an obstacle to
the production of soluble recombinant proteins. We present a general method to assess the …

NMR spectroscopy and X-ray crystallography, the two primary experimental methods for
protein structure determination at high resolution, have different advantages and …