The proteasome degrades most cellular proteins in a controlled and tightly regulated
manner and thereby controls many processes, including cell cycle, transcription, signalling …

Next-generation DNA sequencing technologies have led to a massive accumulation of
genomic and transcriptomic data from patients and healthy individuals. The major challenge …

The mechanisms by which cells adapt to proteotoxic stress are largely unknown, but are key
to understanding how tumor cells, particularly in vivo, are largely resistant to proteasome …

CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome
function, but can be confounded by nuclease-induced toxicity at both on-and off-target sites …

We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify
essential genes in the human chronic myelogenous leukemia cell line K562. We found that …

Proteasomes are essential for protein homeostasis in eukaryotes. To preserve cellular
function, transcription of proteasome subunit genes is induced in response to proteasome …

Abstract Hop/Stip1/Sti1 is thought to be essential as a co-chaperone to facilitate substrate
transfer between the Hsp70 and Hsp90 molecular chaperones. Despite this proposed key …

To achieve maximal growth, cells must manage a massive economy of ribosomal proteins (r-
proteins) and RNAs (rRNAs) to produce thousands of ribosomes every minute. Although …

Protein misfolding is a major driver of ageing‐associated frailty and disease pathology.
Although all cells possess multiple, well‐characterised protein quality control systems to …

The Golgi is a dynamic organelle whose correct assembly is crucial for cellular homeostasis.
Perturbations in Golgi structure are associated with numerous disorders from …