Constraining the metabolic genotype–phenotype relationship using a phylogeny of in silico methods

NE Lewis, H Nagarajan, BO Palsson - Nature Reviews Microbiology, 2012 - nature.com
Reconstructed microbial metabolic networks facilitate a mechanistic description of the
genotype–phenotype relationship through the deployment of constraint-based …

An evolutionary perspective on the Crabtree effect

T Pfeiffer, A Morley - Frontiers in molecular biosciences, 2014 - frontiersin.org
The capability to ferment sugars into ethanol is a key metabolic trait of yeasts. Crabtree-
positive yeasts use fermentation even in the presence of oxygen, where they could, in …

A protocol for generating a high-quality genome-scale metabolic reconstruction

I Thiele, BØ Palsson - Nature protocols, 2010 - nature.com
Network reconstructions are a common denominator in systems biology. Bottom–up
metabolic network reconstructions have been developed over the last 10 years. These …

Glycolytic strategy as a tradeoff between energy yield and protein cost

A Flamholz, E Noor, A Bar-Even… - Proceedings of the …, 2013 - National Acad Sciences
Contrary to the textbook portrayal of glycolysis as a single pathway conserved across all
domains of life, not all sugar-consuming organisms use the canonical Embden–Meyerhoff …

Omic data from evolved E. coli are consistent with computed optimal growth from genome‐scale models

NE Lewis, KK Hixson, TM Conrad… - Molecular systems …, 2010 - embopress.org
After hundreds of generations of adaptive evolution at exponential growth, Escherichia coli
grows as predicted using flux balance analysis (FBA) on genome‐scale metabolic models …

Shifts in growth strategies reflect tradeoffs in cellular economics

D Molenaar, R Van Berlo, D De Ridder… - Molecular systems …, 2009 - embopress.org
The growth rate‐dependent regulation of cell size, ribosomal content, and metabolic
efficiency follows a common pattern in unicellular organisms: with increasing growth rates …

Upgrading liquor-making wastewater into medium chain fatty acid: insights into co-electron donors, key microflora, and energy harvest

Q Wu, W Guo, X Bao, X Meng, R Yin, J Du, H Zheng… - Water research, 2018 - Elsevier
Ethanol and lactate are considered suitable electron donors (EDs) for chain elongation (CE);
however, their respective shortcomings still limit the substrate conversion ratio and medium …

Pathway thermodynamics highlights kinetic obstacles in central metabolism

E Noor, A Bar-Even, A Flamholz, E Reznik… - PLoS computational …, 2014 - journals.plos.org
In metabolism research, thermodynamics is usually used to determine the directionality of a
reaction or the feasibility of a pathway. However, the relationship between thermodynamic …

Flux balance analysis of cyanobacterial metabolism: the metabolic network of Synechocystis sp. PCC 6803

H Knoop, M Gründel, Y Zilliges… - PLoS computational …, 2013 - journals.plos.org
Cyanobacteria are versatile unicellular phototrophic microorganisms that are highly
abundant in many environments. Owing to their capability to utilize solar energy and …

Network context and selection in the evolution to enzyme specificity

H Nam, NE Lewis, JA Lerman, DH Lee, RL Chang… - Science, 2012 - science.org
Enzymes are thought to have evolved highly specific catalytic activities from promiscuous
ancestral proteins. By analyzing a genome-scale model of Escherichia coli metabolism, we …