The expanding world of tRNA modifications and their disease relevance

T Suzuki - Nature Reviews Molecular Cell Biology, 2021 - nature.com
Transfer RNA (tRNA) is an adapter molecule that links a specific codon in mRNA with its
corresponding amino acid during protein synthesis. tRNAs are enzymatically modified post …

tRNA therapeutics for genetic diseases

J Coller, Z Ignatova - Nature Reviews Drug Discovery, 2024 - nature.com
Transfer RNAs (tRNAs) have a crucial role in protein synthesis, and in recent years, their
therapeutic potential for the treatment of genetic diseases—primarily those associated with a …

Engineered tRNAs suppress nonsense mutations in cells and in vivo

S Albers, EC Allen, N Bharti, M Davyt, D Joshi… - Nature, 2023 - nature.com
Nonsense mutations are the underlying cause of approximately 11% of all inherited genetic
diseases. Nonsense mutations convert a sense codon that is decoded by tRNA into a …

Transfer RNA modifications and cellular thermotolerance

T Ohira, T Suzuki - Molecular Cell, 2024 - cell.com
RNA molecules are modified post-transcriptionally to acquire their diverse functions.
Transfer RNA (tRNA) has the widest variety and largest numbers of RNA modifications …

Translation velocity determines the efficacy of engineered suppressor tRNAs on pathogenic nonsense mutations

N Bharti, L Santos, M Davyt, S Behrmann… - Nature …, 2024 - nature.com
Nonsense mutations–the underlying cause of approximately 11% of all genetic diseases–
prematurely terminate protein synthesis by mutating a sense codon to a premature stop or …

Repurposing tRNAs for nonsense suppression

S Albers, B Beckert, MC Matthies, CS Mandava… - Nature …, 2021 - nature.com
Three stop codons (UAA, UAG and UGA) terminate protein synthesis and are almost
exclusively recognized by release factors. Here, we design de novo transfer RNAs (tRNAs) …

Ribosomal incorporation of negatively charged d-α- and N-methyl-l-α-amino acids enhanced by EF-Sep

T Katoh, H Suga - … Transactions of the Royal Society B, 2023 - royalsocietypublishing.org
Ribosomal incorporation of d-α-amino acids (dAA) and N-methyl-l-α-amino acids (MeAA)
with negatively charged sidechains, such as d-Asp, d-Glu, MeAsp and MeGlu, into nascent …

Expansion of the genetic code through reassignment of redundant sense codons using fully modified tRNA

CAL McFeely, KK Dods, SS Patel… - Nucleic Acids …, 2022 - academic.oup.com
Breaking codon degeneracy for the introduction of non-canonical amino acids offers many
opportunities in synthetic biology. Yet, despite the existence of 64 codons, the code has only …

Tuning tRNAs for improved translation

JL Weiss, JC Decker, A Bolano, N Krahn - Frontiers in Genetics, 2024 - frontiersin.org
Transfer RNAs have been extensively explored as the molecules that translate the genetic
code into proteins. At this interface of genetics and biochemistry, tRNAs direct the efficiency …

Posttranscriptional modification to the core of tRNAs modulates translational misreading errors

S Saleh, PJ Farabaugh - RNA, 2024 - rnajournal.cshlp.org
Protein synthesis on the ribosome involves successive rapid recruitment of cognate
aminoacyl-tRNAs and rejection of the much more numerous incorrect near-or non-cognates …