How far are non-viral vectors to come of age and reach clinical translation in gene therapy?

M Sainz-Ramos, I Gallego, I Villate-Beitia… - International Journal of …, 2021 - mdpi.com
Efficient delivery of genetic material into cells is a critical process to translate gene therapy
into clinical practice. In this sense, the increased knowledge acquired during past years in …

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and …

G Gauglitz - Analytical and Bioanalytical Chemistry, 2020 - Springer
Direct optical detection has proven to be a highly interesting tool in biomolecular interaction
analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis …

Comparison of the binding of reversible inhibitors to human butyrylcholinesterase and acetylcholinesterase: A crystallographic, kinetic and calorimetric study

TL Rosenberry, X Brazzolotto, IR Macdonald… - Molecules, 2017 - mdpi.com
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) hydrolyze the
neurotransmitter acetylcholine and, thereby, function as coregulators of cholinergic …

[HTML][HTML] Overview of the detection methods for equilibrium dissociation constant KD of drug-receptor interaction

W Ma, L Yang, L He - Journal of pharmaceutical analysis, 2018 - Elsevier
Drug-receptor interaction plays an important role in a series of biological effects, such as cell
proliferation, immune response, tumor metastasis, and drug delivery. Therefore, the …

Assays and technologies for develo** proteolysis targeting chimera degraders

X Liu, X Zhang, D Lv, Y Yuan, G Zheng… - Future Medicinal …, 2020 - Taylor & Francis
Targeted protein degradation by small-molecule degraders represents an emerging mode of
action in drug discovery. Proteolysis targeting chimeras (PROTACs) are small molecules …

Uncertainty in protein–ligand binding constants: asymmetric confidence intervals versus standard errors

V Paketurytė, V Petrauskas, A Zubrienė… - European Biophysics …, 2021 - Springer
Equilibrium binding constants (K b) between chemical compounds and target proteins or
between interacting proteins provide a quantitative understanding of biological interaction …

Combined NMR and Isothermal Titration Calorimetry Investigation Resolves Conditions for Ligand Exchange and Phase Transformation in CsPbBr3 Nanocrystals

SL Abiodun, MY Gee, AB Greytak - The Journal of Physical …, 2021 - ACS Publications
Inorganic halide perovskite nanocrystals (NCs), such as CsPbBr3 quantum dots, have
emerged as an intriguing alternative to traditional semiconductors in optoelectronic devices …

Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020

RJ Falconer, B Schuur… - Journal of Molecular …, 2021 - Wiley Online Library
The last 5 years have seen a series of advances in the application of isothermal titration
microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in …

Isothermal titration calorimetry resolves sequential ligand exchange and association reactions in treatment of oleate-capped CdSe quantum dots with alkylphosphonic …

MY Gee, Y Shen, AB Greytak - The Journal of Physical Chemistry …, 2020 - ACS Publications
This article examines a prototypical nanocrystal surface reaction, the replacement of
carboxylate ligands with alkylphosphonate on CdSe quantum dots (QDs), in an anhydrous …

Rational design of thermodynamic and kinetic binding profiles by optimizing surface water networks coating protein-bound ligands

SG Krimmer, J Cramer, M Betz, V Fridh… - Journal of medicinal …, 2016 - ACS Publications
A previously studied congeneric series of thermolysin inhibitors addressing the solvent-
accessible S2′ pocket with different hydrophobic substituents showed modulations of the …