Genetic Encoding of Fluoro-l-tryptophans for Site-Specific Detection of Conformational Heterogeneity in Proteins by NMR Spectroscopy

H Qianzhu, EH Abdelkader, G Otting… - Journal of the American …, 2024 - ACS Publications
The substitution of a single hydrogen atom in a protein by fluorine yields a site-specific
probe for sensitive detection by 19F nuclear magnetic resonance (NMR) spectroscopy …

[HTML][HTML] Non-canonical amino acids for site-directed spin labeling of membrane proteins

K Ledwitch, G Künze, E Okwei, D Sala… - Current Opinion in …, 2024 - Elsevier
Highlights•Genetically encodable ncAAs are expanding our options for spin-labeling
biomolecules.•Conventional cysteine-based SDSL schemes can complicate biophysical …

Spin labels for 19 F ENDOR distance determination: resolution, sensitivity and distance predictability

A Bogdanov, L Gao, A Dalaloyan, W Zhu… - Physical Chemistry …, 2024 - pubs.rsc.org
19F electron-nuclear double resonance (ENDOR) has emerged as an attractive method for
determining distance distributions in biomolecules in the range of 0.7–2 nm, which is not …

Pulsed EPR methods in the angstrom to nanometre scale shed light on the conformational flexibility of a Fluoride Riboswitch

L Remmel, A Meyer, K Ackermann… - Angewandte Chemie …, 2024 - Wiley Online Library
Riboswitches control gene regulation upon external stimuli such as environmental factors or
ligand binding. The fluoride sensing riboswitch from Thermotoga petrophila is a complex …

Fluorinated Tags to Study Protein Conformation and Interactions Using 19F NMR

GSM Hanson, CR Coxon - ChemBioChem, 2024 - Wiley Online Library
The incorporation of fluorine atoms into a biomacromolecule provides a background‐free
and environmentally sensitive reporter of structure, conformation and interactions using 19F …

Frequency and time domain 19 F ENDOR spectroscopy: role of nuclear dipolar couplings to determine distance distributions

A Kehl, L Sielaff, L Remmel, ML Rämisch… - Physical Chemistry …, 2025 - pubs.rsc.org
19F electron-nuclear double resonance (ENDOR) spectroscopy is emerging as a method of
choice to determine molecular distances in biomolecules in the angstrom to nanometer …

Host–guest geometry in paramagnetic cavitands elucidated by 19 F electron-nuclear double resonance

A Bogdanov, M Seal, E Goren, A Bar-Shir… - Physical Chemistry …, 2025 - pubs.rsc.org
Elucidating structural information of supramolecular host–guest systems is pivotal for
understanding molecular recognition and designing functional materials. This study …

Puls EPR Methoden im Angstöm‐bis Nanometerbereich geben Aufschluss über die konformationelle Flexibilität eines Fluorid‐Riboschalters

L Remmel, A Meyer, K Ackermann… - Angewandte …, 2024 - Wiley Online Library
Riboschalter steuern die Genregulation in Reaktion auf externe Reize wie Umweltfaktoren
oder Ligandenbindung. Der fluoriderkennende Riboschalter aus Thermotoga petrophila ist …

[PDF][PDF] Pulse EPR Methods in the Angstrom to Nanometre Scale Shed Light on the Conformational Flexibility of a Fluoride Riboswitch

L Remmel, A Meyer, K Ackermann… - Angewandte Chemie …, 2024 - pure.mpg.de
Riboswitches control gene regulation upon external stimuli such as environmental factors or
ligand binding. The fluoride sensing riboswitch from Thermotoga petrophila is a complex …