The evolution of insect biodiversity

E Tihelka, C Cai, M Giacomelli, J Lozano-Fernandez… - Current Biology, 2021 - cell.com
Insects comprise over half of all described animal species. Together with the Protura
(coneheads), Collembola (springtails) and Diplura (two-pronged bristletails), insects form …

The mechanisms of substrate selection, catalysis, and translocation by the elongating RNA polymerase

GA Belogurov, I Artsimovitch - Journal of molecular biology, 2019 - Elsevier
Multi-subunit DNA-dependent RNA polymerases synthesize all classes of cellular RNAs,
ranging from short regulatory transcripts to gigantic messenger RNAs. RNA polymerase has …

Trigger loop of RNA polymerase is a positional, not acid–base, catalyst for both transcription and proofreading

TV Mishanina, MZ Palo, D Nayak, RA Mooney… - Proceedings of the …, 2017 - pnas.org
The active site of multisubunit RNA polymerases (RNAPs) is highly conserved from humans
to bacteria. This single site catalyzes both nucleotide addition required for RNA transcript …

Dual Role of a (p) ppGpp‐and (p) ppApp‐degrading Enzyme in Biofilm Formation and Interbacterial Antagonism

W Steinchen, S Ahmad, M Valentini… - Molecular …, 2021 - Wiley Online Library
The guanosine nucleotide‐based second messengers ppGpp and pppGpp (collectively:(p)
ppGpp) enable adaptation of microorganisms to environmental changes and stress …

High-resolution phenotypic landscape of the RNA polymerase II trigger loop

C Qiu, OC Erinne, JM Dave, P Cui, H **… - PLoS …, 2016 - journals.plos.org
The active sites of multisubunit RNA polymerases have a “trigger loop”(TL) that multitasks in
substrate selection, catalysis, and translocation. To dissect the Saccharomyces cerevisiae …

Two distinct pathways of RNA polymerase backtracking determine the requirement for the Trigger Loop during RNA hydrolysis

H Mosaei, N Zenkin - Nucleic acids research, 2021 - academic.oup.com
Transcribing RNA polymerase (RNAP) can fall into backtracking, phenomenon when the 3′
end of the transcript disengages from the template DNA. Backtracking is caused by …

[HTML][HTML] Transcription fidelity and its roles in the cell

P Gamba, N Zenkin - Current Opinion in Microbiology, 2018 - Elsevier
Highlights•The Trigger Loop is one of the major determinants of transcription fidelity.•Intrinsic
proofreading occurs via transcript-assisted cleavage.•Factor-assisted proofreading takes …

Distinct effects of DNA lesions on RNA synthesis by Escherichia coli RNA polymerase

D Pupov, A Ignatov, A Agapov… - … and Biophysical Research …, 2019 - Elsevier
DNA lesions can severely compromise genome stability and lead to cell death if unrepaired.
RNA polymerase (RNAP) is known to serve as a sensor of DNA damage and to attract DNA …

Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock

E Klimuk, E Bogdanova, M Nagornykh… - Nucleic acids …, 2018 - academic.oup.com
C-proteins control restriction–modification (R–M) systems' genes transcription to ensure
sufficient levels of restriction endonuclease to allow protection from foreign DNA while …

[HTML][HTML] Kinetic Features of Degradation of R-Loops by RNase H1 from Escherichia coli

AA Kuznetsova, IA Kosarev, NA Timofeyeva… - International Journal of …, 2024 - mdpi.com
R-loops can act as replication fork barriers, creating transcription–replication collisions and
inducing replication stress by arresting DNA synthesis, thereby possibly causing aberrant …