Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020‏ - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward

M Yao, S Zhang, Q Lu, X Chen, SY Zhang… - Molecular …, 2022‏ - Wiley Online Library
Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …

[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022‏ - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020‏ - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

The current state of eDNA research in freshwater ecosystems: are we shifting from the developmental phase to standard application in biomonitoring?

T Schenekar - Hydrobiologia, 2023‏ - Springer
Environmental DNA (eDNA) has seen a massive increase in application in freshwater
systems with a concurrent growth in protocol developments and a drive to gain a better …

Improving the reliability of eDNA data interpretation

A Burian, Q Mauvisseau, M Bulling… - Molecular Ecology …, 2021‏ - Wiley Online Library
Global declines in biodiversity highlight the need to effectively monitor the density and
distribution of threatened species. In recent years, molecular survey methods detecting DNA …

Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary

G Kumar, AM Reaume, E Farrell, MR Gaither - PloS one, 2022‏ - journals.plos.org
Metabarcoding of environmental DNA is increasingly used for biodiversity assessments in
aquatic communities. The efficiency and outcome of these efforts are dependent upon either …

An integrated spatio-temporal view of riverine biodiversity using environmental DNA metabarcoding

WB Perry, M Seymour, L Orsini, IB Jâms… - Nature …, 2024‏ - nature.com
Anthropogenically forced changes in global freshwater biodiversity demand more efficient
monitoring approaches. Consequently, environmental DNA (eDNA) analysis is enabling …

Biodiversity assessment across a dynamic riverine system: A comparison of eDNA metabarcoding versus traditional fish surveying methods

J Hallam, EL Clare, JI Jones, JJ Day - Environmental DNA, 2021‏ - Wiley Online Library
While many studies have considered the ability of eDNA to assess animal communities in
lacustrine settings, fewer have considered riverine systems, particularly those spanning the …

Current status and topical issues on the use of eDNA-based targeted detection of rare animal species

S Duarte, L Simões, FO Costa - Science of the Total Environment, 2023‏ - Elsevier
Animal detection through DNA present in environmental samples (eDNA) is a valuable tool
for detecting rare species, that are difficult to observe and monitor. eDNA-based tools are …