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Advances in promoter engineering: novel applications and predefined transcriptional control
Synthetic biology continues to progress by relying on more robust tools for transcriptional
control, of which promoters are the most fundamental component. Numerous studies have …
control, of which promoters are the most fundamental component. Numerous studies have …
De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts
Y Xu, X Wang, C Zhang, X Zhou, X Xu, L Han… - Nature …, 2022 - nature.com
High-sugar diet causes health problems, many of which can be addressed with the use of
sugar substitutes. Rubusoside and rebaudiosides are interesting molecules, considered the …
sugar substitutes. Rubusoside and rebaudiosides are interesting molecules, considered the …
GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER
Tail-anchored (TA) membrane proteins have a potential risk to be mistargeted to the
mitochondrial outer membrane (OM). Such mislocalized TA proteins can be extracted by the …
mitochondrial outer membrane (OM). Such mislocalized TA proteins can be extracted by the …
A layered genetic design enables the yeast galactose regulon to respond to cyanamide
The commonly used expression systems in Saccharomyces cerevisiae typically rely on
either constitutive or galactose-regulated promoters. The lack of inducible systems in S …
either constitutive or galactose-regulated promoters. The lack of inducible systems in S …
Alleviation of the Byproducts Formation Enables Highly Efficient Biosynthesis of Rosmarinic Acid in Saccharomyces cerevisiae
P Zhou, C Yue, Y Zhang, Y Li, X Da… - Journal of Agricultural …, 2022 - ACS Publications
Rosmarinic acid as a polyphenolic compound has great values in the pharmaceutical,
cosmetic, and food industries. To achieve efficient biosynthesis of rosmarinic acid, the major …
cosmetic, and food industries. To achieve efficient biosynthesis of rosmarinic acid, the major …
Photothermally modulated magnetic nanochains as swarm nanorobotics for microreaction control
The intensification of microreactions is important for biological and chemical reactions with
microliter volumes as reaction units, including microliter bioassay or protein synthesis. At the …
microliter volumes as reaction units, including microliter bioassay or protein synthesis. At the …
[HTML][HTML] Cyanamide-inducible expression of homing nuclease I− SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae
In synthetic biology, microbial chassis including yeast Saccharomyces cerevisiae are
iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering …
iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering …
Assigning roles in Chlamydomonas ribosome biogenesis: The conserved factor NIP7
Ribosome biogenesis (RB) is a highly conserved process across eukaryotes that results in
the assembly of functional ribosomal subunits. Studies in Saccharomyces cerevisiae and …
the assembly of functional ribosomal subunits. Studies in Saccharomyces cerevisiae and …
Uniform [13C,15N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae
Despite the prevalence and importance of glycoproteins in human biology, methods for
isotope labeling suffer significant limitations. Common prokaryotic platforms do not produce …
isotope labeling suffer significant limitations. Common prokaryotic platforms do not produce …
Cyanamide-inducible expression of homing nuclease I-SceI for iterative genome engineering and parallel promoter characterisation in Saccharomyces cerevisiae
In synthetic biology, microbial chasses including yeast Saccharomyces cerevisiae are
iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering …
iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering …