Since their serendipitous discovery in nematodes, microRNAs (miRNAs) have emerged as
key regulators of biological processes in animals. These small RNAs form complex networks …

MicroRNAs (miRNAs) are versatile, post-transcriptional regulators of gene expression.
Canonical miRNAs are generated through the two-step DROSHA-and DICER-mediated …

The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has
been demonstrated in a multitude of different in vitro cell systems and in a variety of body …

Non-coding RNAs (ncRNAs) are considered major players in physiological and pathological
processes based on their versatile regulatory roles in different diseases including …

MicroRNAs (miRNAs) broadly regulate gene expression through association with Argonaute
(Ago), which also protects miRNAs from degradation. However, miRNA stability is known to …

Summary miRISC is a multi-protein assembly that uses microRNAs (miRNAs) to identify
mRNAs targeted for repression. Dozens of miRISC-associated proteins have been …

Therapeutic small interfering RNA (siRNA) requires sugar and backbone modifications to
inhibit nuclease degradation. However, metabolic stabilization by phosphorothioate (PS) …

Hundreds of microRNAs (miRNAs) are expressed in distinct spatial and temporal patterns
during embryonic and postnatal mouse development. The loss of all miRNAs through the …

Argonaute is a key enzyme of various RNA silencing pathways. We use single-molecule
fluorescence measurements to characterize the reaction mechanisms of the core-RISC …

Little is known about miRNA decay. A target-directed miRNA degradation mechanism
(TDMD) has been suggested, but further investigation on endogenous targets is necessary …