Single-molecule localization microscopy in eukaryotes

M Sauer, M Heilemann - Chemical reviews, 2017 - ACS Publications
Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Theory and simulation of the ultrafast double-bond isomerization of biological chromophores

S Gozem, HL Luk, I Schapiro, M Olivucci - Chemical reviews, 2017 - ACS Publications
Ultrafast processes in light-absorbing proteins have been implicated in the primary step in
the light-to-energy conversion and the initialization of photoresponsive biological functions …

Fluorescent proteins and their applications in imaging living cells and tissues

DM Chudakov, MV Matz, S Lukyanov… - Physiological …, 2010 - journals.physiology.org
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

A guide to choosing fluorescent proteins

NC Shaner, PA Steinbach, RY Tsien - Nature methods, 2005 - nature.com
The recent explosion in the diversity of available fluorescent proteins (FPs),,,,,,,,,,,,,,,
promises a wide variety of new tools for biological imaging. With no unified standard for …

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging

GT Dempsey, JC Vaughan, KH Chen, M Bates… - Nature …, 2011 - nature.com
One approach to super-resolution fluorescence imaging uses sequential activation and
localization of individual fluorophores to achieve high spatial resolution. Essential to this …

Switchable solvatochromic probes for live‐cell super‐resolution imaging of plasma membrane organization

DI Danylchuk, S Moon, K Xu… - Angewandte …, 2019 - Wiley Online Library
Visualization of the nanoscale organization of cell membranes remains challenging
because of the lack of appropriate fluorescent probes. Herein, we introduce a new design …

The fluorescent protein palette: tools for cellular imaging

RN Day, MW Davidson - Chemical Society Reviews, 2009 - pubs.rsc.org
This critical review provides an overview of the continually expanding family of fluorescent
proteins (FPs) that have become essential tools for studies of cell biology and physiology …

Photoinduced chemistry in fluorescent proteins: curse or blessing?

A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Principles of two-photon excitation microscopy and its applications to neuroscience

K Svoboda, R Yasuda - Neuron, 2006 - cell.com
The brain is complex and dynamic. The spatial scales of interest to the neurobiologist range
from individual synapses (∼ 1 μm) to neural circuits (centimeters); the timescales range from …