Methods to improve the accuracy of next-generation sequencing

C Cheng, Z Fei, P **ao - Frontiers in bioengineering and …, 2023 - frontiersin.org
Next-generation sequencing (NGS) is present in all fields of life science, which has greatly
promoted the development of basic research while being gradually applied in clinical …

[HTML][HTML] Next-generation sequencing in high-sensitive detection of mutations in tumors: challenges, advances, and applications

RR Singh - The Journal of Molecular Diagnostics, 2020 - Elsevier
Next-generation sequencing (NGS) technologies have come of age as preferred
technologies for screening of genomic variants of pathologic and therapeutic potential …

UMI-tools: modeling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy

T Smith, A Heger, I Sudbery - Genome research, 2017 - genome.cshlp.org
Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are
increasingly used in high-throughput sequencing experiments. Through a UMI, identical …

Detecting ultralow-frequency mutations by Duplex Sequencing

SR Kennedy, MW Schmitt, EJ Fox, BF Kohrn, JJ Salk… - Nature protocols, 2014 - nature.com
Duplex Sequencing (DS) is a next-generation sequencing methodology capable of
detecting a single mutation among> 1× 107 wild-type nucleotides, thereby enabling the …

Detection of ultra-rare mutations by next-generation sequencing

MW Schmitt, SR Kennedy, JJ Salk, EJ Fox… - Proceedings of the …, 2012 - pnas.org
Next-generation DNA sequencing promises to revolutionize clinical medicine and basic
research. However, while this technology has the capacity to generate hundreds of billions …

Detection and quantification of rare mutations with massively parallel sequencing

I Kinde, J Wu, N Papadopoulos, KW Kinzler… - Proceedings of the …, 2011 - pnas.org
The identification of mutations that are present in a small fraction of DNA templates is
essential for progress in several areas of biomedical research. Although massively parallel …

Accuracy of next generation sequencing platforms

EJ Fox, KS Reid-Bayliss, MJ Emond… - Next generation …, 2014 - pmc.ncbi.nlm.nih.gov
Next-generation DNA sequencing has revolutionized genomic studies and is driving the
implementation of precision diagnostics. The ability of these technologies to disentangle …

Nucleic acid constructs and methods of use

MS Chee - US Patent 9,783,847, 2017 - Google Patents
The present invention provides oligonucleotide constructs, sets of such oligonucleotide
constructs, and methods of using such oligonucleotide constructs to provide validated …

Spatially encoded biological assays

MS Chee - US Patent 10,308,982, 2019 - Google Patents
The present invention provides assays and assay systems for use in spatially encoded
biological assays. The invention provides an assay system comprising an assay capable of …

Methods for non-invasive prenatal ploidy calling

M Rabinowitz, G Gemelos, M Banjevic, A Ryan… - US Patent …, 2018 - Google Patents
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