Efficient and feasible biocatalysts: Strategies for enzyme improvement. A review
Owing to the environmental friendliness and vast advantages that enzymes offer in the
biotechnology and industry fields, biocatalysts are a prolific investigation field. However, the …
biotechnology and industry fields, biocatalysts are a prolific investigation field. However, the …
In-Depth Sequence–Function Characterization Reveals Multiple Pathways to Enhance Enzymatic Activity
Deep mutational scanning (DMS) has recently emerged as a powerful method to study
protein sequence–function relationships but is not well-explored as a guide to enzyme …
protein sequence–function relationships but is not well-explored as a guide to enzyme …
Characterization of Phenylalanine Ammonia Lyases from Lettuce (Lactuca sativa L.) as Robust Biocatalysts for the Production of d- and l-Amino Acids
BF Zhu, Y Liu, XQ Pei, ZL Wu - Journal of Agricultural and Food …, 2023 - ACS Publications
Phenylalanine ammonia lyase (PAL) catalyzes the reversible conversion of l-phenylalanine
into the corresponding trans-cinnamic acid, providing a route to optically pure α-amino …
into the corresponding trans-cinnamic acid, providing a route to optically pure α-amino …
Engineered Zea mays phenylalanine ammonia-lyase for improve the catalytic efficiency of biosynthesis trans-cinnamic acid and p-coumaric acid
J Zheng, R Sun, D Wu, P Chen, P Zheng - Enzyme and Microbial …, 2024 - Elsevier
Phenylalanine ammonia-lyase (PAL) plays a pivotal role in the biosynthesis of
phenylalanine. PAL from Zea mays (ZmPAL2) exhibits a bi-function of direct deamination of …
phenylalanine. PAL from Zea mays (ZmPAL2) exhibits a bi-function of direct deamination of …
Phenylalanine ammonia-lyases: combining protein engineering and natural diversity
In this study, rational design and saturation mutagenesis efforts for engineering
phenylalanine ammonia-lyase from Petroselinum crispum (Pc PAL) provided tailored PALs …
phenylalanine ammonia-lyase from Petroselinum crispum (Pc PAL) provided tailored PALs …
Robust, site-specifically immobilized phenylalanine ammonia-lyases for the enantioselective ammonia addition of cinnamic acids
Phenylalanine ammonia-lyases (PALs) catalyse the non-oxidative deamination of L-
phenylalanine to trans-cinnamic acid, while in the presence of high ammonia concentration …
phenylalanine to trans-cinnamic acid, while in the presence of high ammonia concentration …
Engineering phenylalanine ammonia lyase to limit feedback inhibition by cinnamate and enhance biotransformation
Phenylalanine ammonia‐lyase (PAL) is a crucial enzyme for various biotechnology
applications, such as producing phenols, antioxidants, and nutraceuticals. However …
applications, such as producing phenols, antioxidants, and nutraceuticals. However …
Engineered, Scalable Production of Optically Pure l-Phenylalanines Using Phenylalanine Ammonia-Lyase from Arabidopsis thaliana
An efficient preparative-scale synthetic procedure of l-phenylalanine derivatives has been
developed using mutant variants of phenylalanine ammonia-lyase from Arabidopsis thaliana …
developed using mutant variants of phenylalanine ammonia-lyase from Arabidopsis thaliana …
Fluorescent enzyme-coupled activity assay for phenylalanine ammonia-lyases
ME Moisă, DA Amariei, EZA Nagy, N Szarvas… - Scientific Reports, 2020 - nature.com
Phenylalanine ammonia-lyases (PALs) catalyse the non-oxidative deamination of l-
phenylalanine to trans-cinnamic acid, while in the presence of high ammonia concentration …
phenylalanine to trans-cinnamic acid, while in the presence of high ammonia concentration …
Ancestral l-amino acid oxidase: From substrate scope exploration to phenylalanine ammonia-lyase assay
In this study we assessed the applicability of the recently reported ancestral l-amino acid
oxidase (AncLAAO), for the development of an enzyme-coupled phenylalanine ammonia …
oxidase (AncLAAO), for the development of an enzyme-coupled phenylalanine ammonia …