Agrobacterium-mediated plant transformation: biology and applications

HH Hwang, M Yu, EM Lai - The arabidopsis book, 2017‏ - pmc.ncbi.nlm.nih.gov
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …

[HTML][HTML] Current progress and challenges in crop genetic transformation

RB Anjanappa, W Gruissem - Journal of Plant Physiology, 2021‏ - Elsevier
Plant transformation remains the most sought-after technology for functional genomics and
crop genetic improvement, especially for introducing specific new traits and to modify or …

[HTML][HTML] The present and potential future methods for delivering CRISPR/Cas9 components in plants

D Sandhya, P Jogam, VR Allini, S Abbagani… - Journal of Genetic …, 2020‏ - Elsevier
Background CRISPR/Cas9 genome editing technology is a DNA manipulation tool for trait
improvement. This technology has been demonstrated and successfully applied to edit the …

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

V Verma, A Kumar, M Partap, M Thakur… - Frontiers in plant …, 2023‏ - frontiersin.org
The acceptance of new crop varieties by consumers is contingent on the presence of
consumer-preferred traits, which include sensory attributes, nutritional value, industrial …

Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat

H Hamada, Y Liu, Y Nagira, R Miki, N Taoka, R Imai - Scientific reports, 2018‏ - nature.com
The current application of genome editing to crop plants is limited to cultivars that are
amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing …

An in planta biolistic method for stable wheat transformation

H Hamada, Q Linghu, Y Nagira, R Miki, N Taoka… - Scientific reports, 2017‏ - nature.com
The currently favoured method for wheat (Triticum aestivum L.) transformation is
inapplicable to many elite cultivars because it requires callus culture and regeneration …

Strategies for high-level recombinant protein expression in transgenic microalgae: a review

G Potvin, Z Zhang - Biotechnology advances, 2010‏ - Elsevier
Microalgae represent the 'best of both worlds', combining the high growth rate and ease of
cultivation of microorganisms with the ability to perform post-transcriptional and post …

Genetic modification for wheat improvement: from transgenesis to genome editing

N Borisjuk, O Kishchenko, S Eliby… - BioMed Research …, 2019‏ - Wiley Online Library
To feed the growing human population, global wheat yields should increase to
approximately 5 tonnes per ha from the current 3.3 tonnes by 2050. To reach this goal …

Efficient Agrobacterium transformation of elite wheat germplasm without selection

T Richardson, J Thistleton, TJ Higgins, C Howitt… - Plant Cell, Tissue and …, 2014‏ - Springer
A previously reported Agrobacterium tumefaciens transformation system that transformed
wheat cultivar Fielder at high efficiency was shown to also transform eight out of nine …

[PDF][PDF] Tissue culture-based Agrobacterium-mediated and in planta transformation methods.

M Niazian, SAS Noori, P Galuszka, SMM Mortazavian - 2017‏ - agriculturejournals.cz
Abstract Niazian M., Sadat Noori SA, Galuszka P., Mortazavian SMM (2017): Tissue culture-
based Agrobacterium-mediated and in planta transformation methods. Czech J. Genet. Plant …