Pyridoxal 5′-phosphate (PLP) functions as a coenzyme in many enzymatic processes,
including decarboxylation, deamination, transamination, racemization, and others. Enzymes …

Penicillium chrysogenum (renamed P. rubens) is the most studied member of a family of
more than 350 Penicillium species that constitute the genus. Since the discovery of penicillin …

Biosensors are powerful tools for detecting, real-time imaging, and quantifying molecules,
but rapidly constructing diverse genetically encoded biosensors remains challenging. Here …

Nonribosomal peptides are a prolific source of bioactive molecules biosynthesized on large,
modular assembly line synthetases. Synthetic biologists seek to obtain tailored peptides with …

Nonribosomal peptide synthetases (NRPS) are large multimodular enzymes that synthesize
a diverse variety of peptides. Many of these are currently used as pharmaceuticals, thanks to …

Nonribosomal peptide synthetases (NRPSs) are multidomain enzyme templates for the
synthesis of bioactive peptides. Large-scale conformational changes during peptide …

Many peptide-derived natural products are produced by non-ribosomal peptide synthetases
(NRPSs) in an assembly-line fashion. Each amino acid is coupled to a designated peptidyl …

l-Enduracididine (l-End) is a nonproteinogenic amino acid found in a number of bioactive
peptides, including the antibiotics teixobactin, enduracidin, and mannopeptimycin. The …

Nonribosomal peptide synthetases (NRPSs) are multifunctional megaenzymes that govern
the stepwise biosynthesis of pharmaceutically important peptides. In an ATP-dependent …

In vitro reconstitution and biochemical analysis of natural product biosynthetic pathways
remains a challenging endeavor, especially if megaenzymes of the nonribosomal peptide …