Second harmonic generation microscopy: a powerful tool for bio-imaging

A Aghigh, S Bancelin, M Rivard, M Pinsard… - Biophysical …, 2023‏ - Springer
Second harmonic generation (SHG) microscopy is an important optical imaging technique in
a variety of applications. This article describes the history and physical principles of SHG …

Consensus guidelines for the use and interpretation of angiogenesis assays

P Nowak-Sliwinska, K Alitalo, E Allen, A Anisimov… - Angiogenesis, 2018‏ - Springer
The formation of new blood vessels, or angiogenesis, is a complex process that plays
important roles in growth and development, tissue and organ regeneration, as well as …

Ly6Clo monocytes drive immunosuppression and confer resistance to anti-VEGFR2 cancer therapy

K Jung, T Heishi, OF Khan, PS Kowalski, J Incio… - The Journal of clinical …, 2017‏ - jci.org
Current anti-VEGF therapies for colorectal cancer (CRC) provide limited survival benefit, as
tumors rapidly develop resistance to these agents. Here, we have uncovered an …

Cancer-associated angiogenesis: the endothelial cell as a checkpoint for immunological patrolling

AG Solimando, SD Summa, A Vacca, D Ribatti - Cancers, 2020‏ - mdpi.com
Simple Summary A clinical decision and study design investigating the level and extent of
angiogenesis modulation aimed at vascular normalization without rendering tissues hypoxic …

Imaging cell biology in live animals: ready for prime time

R Weigert, N Porat-Shliom… - Journal of Cell Biology, 2013‏ - rupress.org
Time-lapse fluorescence microscopy is one of the main tools used to image subcellular
structures in living cells. Yet for decades it has been applied primarily to in vitro model …

Intravital microscopy: a practical guide on imaging intracellular structures in live animals

A Masedunskas, O Milberg, N Porat-Shliom… - …, 2012‏ - Taylor & Francis
Intravital microscopy is an extremely powerful tool that enables imaging several biological
processes in live animals. Recently, the ability to image subcellular structures in several …

Quantitative blood flow estimation in vivo by optical speckle image velocimetry

MM Qureshi, Y Liu, KD Mac, M Kim, AM Safi, E Chung - Optica, 2021‏ - opg.optica.org
<? TeX-2pc-22pc?> Speckle-based methods are popular non-invasive, label-free full-field
optical techniques for imaging blood flow maps at single vessel resolution with a high …

Designing a large field-of-view two-photon microscope using optical invariant analysis

JR Bumstead, JJ Park, IA Rosen, AW Kraft… - …, 2018‏ - spiedigitallibrary.org
Conventional two-photon microscopy (TPM) is capable of imaging neural dynamics with
subcellular resolution, but it is limited to a field-of-view (FOV) diameter< 1 mm. Although …

Multiphoton intravital microscopy of rodents

CLGJ Scheele, D Herrmann, E Yamashita… - Nature Reviews …, 2022‏ - nature.com
Tissues are heterogeneous with respect to cellular and non-cellular components and in the
dynamic interactions between these elements. To study the behaviour and fate of individual …

Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo

YW Choo, J Jeong, K Jung - BMB reports, 2020‏ - pmc.ncbi.nlm.nih.gov
Currently, most biological research relies on conventional experimental techniques that
allow only static analyses at certain time points in vitro or ex vivo. However, if one could …