Trillions of microorganisms inhabit the human body, strongly colonizing the gastro-intestinal
tract and outnumbering our own cells. High-throughput sequencing techniques and new …

The term metagenomics refers to the use of sequencing methods to simultaneously identify
genomic material from all organisms present in a sample, with the advantage of greater …

Background In the last 5 years, the rapid pace of innovations and improvements in
sequencing technologies has completely changed the landscape of metagenomic and …

DNA metabarcoding is a powerful new tool allowing characterization of species
assemblages using high‐throughput amplicon sequencing. The utility of DNA …

The diversity of fungi in grape must and during wine fermentation was investigated in this
study by culture-dependent and culture-independent techniques. Carignan and Grenache …

Ecologists are increasingly interested in quantifying consumer diets based on food DNA in
dietary samples and high‐throughput sequencing of marker genes. It is tempting to assume …

Background Count data derived from high-throughput deoxy-ribonucliec acid (DNA)
sequencing is frequently used in quantitative molecular assays. Due to properties inherent …

Despite the advent of whole genome metagenomics, targeted approaches (such as 16S
rRNA gene amplicon sequencing) continue to be valuable for determining the microbial …

Background Microbiome/host interactions describe characteristics that affect the host's
health. Shotgun metagenomics includes sequencing a random subset of the microbiome to …

The application of high-throughput sequencing in profiling microbial communities is
providing an unprecedented ability to investigate microbiomes. Such studies typically apply …