CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …

Functional genomics approaches can overcome limitations—such as the lack of
identification of robust targets and poor clinical efficacy—that hamper cancer drug …

The proteome provides unique insights into disease biology beyond the genome and
transcriptome. A lack of large proteomic datasets has restricted the identification of new …

Genome-wide pooled CRISPR-Cas-mediated knockout, activation, and repression screens
are powerful tools for functional genomic investigations. Despite their increasing importance …

CRISPR-Cas9 viability screens are increasingly performed at a genome-wide scale across
large panels of cell lines to identify new therapeutic targets for precision cancer therapy …

Genetic screens in cancer cell lines inform gene function and drug discovery. More
comprehensive screen datasets with multi-omics data are needed to enhance opportunities …

Genome-scale CRISPR-Cas9 viability screens performed in cancer cell lines provide a
systematic approach to identify cancer dependencies and new therapeutic targets. As …

Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated
protein 9 (Cas9) is a powerful tool for introducing targeted mutations in DNA, but recent …

Abstract Background Pooled library CRISPR/Cas9 knockout screening across hundreds of
cell lines has identified genes whose disruption leads to fitness defects, a critical step in …