Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

D May, K Paldi, F Altpeter - The Plant Genome, 2023 - Wiley Online Library
Many of the world's most important crops are polyploid. The presence of more than two sets
of chromosomes within their nuclei and frequently aberrant reproductive biology in …

Reduction in Cold-Induced Sweetening by Cas9 Endonuclease-Mediated Knockout of the POTATO VACUOLAR INVERTASE 1 Gene in the Cultivar 'Symfonia'

AA Egorova, TE Zykova, NE Kostina, IA Saboiev… - Potato Research, 2024 - Springer
Storage of potato tubers at low temperatures leads to the degradation of starch into reducing
sugars, resulting in cold-induced sweetening (CIS). These sugars react with free amino …

Fine-tuning CRISPR/Cas9 gene editing in common bean (Phaseolus vulgaris L.) using a hairy root transformation system and in silico prediction models

R de Koning, H Daryanavard, J Garmyn… - Frontiers in Plant …, 2023 - frontiersin.org
The stable transformation of common bean is a challenging and time-consuming process.
Although CRISPR/Cas9 has revolutionized gene editing with its high efficiency and …

Genome editing in species of the tribe Triticeae with the CRISPR/Cas system

BR Kuluev, EV Mikhailova, AR Kuluev, AA Galimova… - Molecular Biology, 2022 - Springer
The tribe Triticeae includes important agricultural crops, such as bread wheat, durum wheat,
barley, rye, and triticale. Research in the field of reverse genetics and genetic engineering of …

Evaluation of CRISPR/Cas9 constructs in wheat cell suspension cultures

K Michalski, P Ziąbska, S Sowa, J Zimny… - International Journal of …, 2023 - mdpi.com
Despite intensive optimization efforts, develo** an efficient sequence-specific
CRISPR/Cas-mediated genome editing method remains a challenge, especially in polyploid …

[PDF][PDF] Somatic embryogenesis, genetic modification, and GMOs at the Department of Plant Biotechnology and Cytogenetics at the IHAR

J Zimny, S Sowa - Acta Societatis Botanicorum Poloniae, 2022 - academia.edu
Abstract At the Plant Breeding and Acclimatization Institute, National Research Institute
(IHAR), research on plant tissue cultures began in 1970s. Some in vitro methods, such as …

[HTML][HTML] Optimized CRISPR/Cas9 system for gene knockout in chicken DF1 cells

K Zou, F Wang, Z Zhang, Y Zhou, P Li, D Wang, M Zhu… - Poultry Science, 2023 - Elsevier
The editing efficiency primarily hinders the utility of Clustered Regularly Interspaced Short
Palindromic Repeats (CRISPR) technology in poultry. For a better understanding of the …

Biosafety issue related to genome editing in plants using CRISPR-Cas9

R Katam, F Hasanvand, V Teniyah, J Noel… - Genome editing: current …, 2022 - Springer
Genetic modification of plants was in practice long before establishing the genetic paradigm.
In their quest to attain superior plants, humans continually modified plants through selection …

[PDF][PDF] Precise Gene Editing of Cereals Using CRISPR/Cas Technology

P Pouramini, G Hensel - A Roadmap for Plant Genome Editing, 2023 - library.oapen.org
Targeted mutagenesis using CRISPR/Cas technology has become routine in elucidating
biological processes or their application in breeding and agriculture. This means that the …

[PDF][PDF] Advances in Triticale (X Triticosecale) Improvement: Chromosome Manipulation and Biotechnological Approaches

M Subedi, SS Sandal, P kulbhushan Savindra… - researchgate.net
Triticale (X Triticosecale) is a hybrid cereal crop with great potential for enhancing food
security. It is a synthetic cereal. Meanwhile, certain genetic instabilities arising from the …