Physical stability of proteins in aqueous solution: mechanism and driving forces in nonnative protein aggregation
Irreversible protein aggregation is problematic in the biotechnology industry, where
aggregation is encountered throughout the lifetime of a therapeutic protein, including during …
aggregation is encountered throughout the lifetime of a therapeutic protein, including during …
Review of the current state of protein aggregation inhibition from a materials chemistry perspective: Special focus on polymeric materials
Protein instability caused by exposure to external additives or severe stress may result in
different diseases. Of these diseases, many are triggered by protein misfolding and …
different diseases. Of these diseases, many are triggered by protein misfolding and …
Biocatalytic transformations in ionic liquids
F Van Rantwijk, RM Lau, RA Sheldon - TRENDS in Biotechnology, 2003 - cell.com
Room temperature ionic liquids are non-volatile, thermally stable and highly polar; they are
also moderately hydrophilic solvents. Here, we discuss their use as reaction media for …
also moderately hydrophilic solvents. Here, we discuss their use as reaction media for …
Role of arginine in protein refolding, solubilization, and purification
K Tsumoto, M Umetsu, I Kumagai, D Ejima… - Biotechnology …, 2004 - Wiley Online Library
Recombinant proteins are often expressed in the form of insoluble inclusion bodies in
bacteria. To facilitate refolding of recombinant proteins obtained from inclusion bodies, 0.1 to …
bacteria. To facilitate refolding of recombinant proteins obtained from inclusion bodies, 0.1 to …
The inverse and direct Hofmeister series for lysozyme
Anion effects on the cloud-point temperature for the liquid− liquid phase transition of
lysozyme were investigated by temperature gradient microfluidics under a dark field …
lysozyme were investigated by temperature gradient microfluidics under a dark field …
The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes
Mitochondria exert an immense amount of cytophysiological functions, but the structural
basis of most of these processes is still poorly understood. Here we use cross-linking mass …
basis of most of these processes is still poorly understood. Here we use cross-linking mass …
High concentration formulation developability approaches and considerations
J Zarzar, T Khan, M Bhagawati, B Weiche… - MAbs, 2023 - Taylor & Francis
The growing need for biologics to be administered subcutaneously and ocularly, coupled
with certain indications requiring high doses, has resulted in an increase in drug substance …
with certain indications requiring high doses, has resulted in an increase in drug substance …
Protein phase behavior in aqueous solutions: crystallization, liquid-liquid phase separation, gels, and aggregates
The aggregates and gels commonly observed during protein crystallization have generally
been considered disordered phases without further characterization. Here their physical …
been considered disordered phases without further characterization. Here their physical …
Ions at aqueous interfaces: From water surface to hydrated proteins
P Jungwirth, B Winter - Annu. Rev. Phys. Chem., 2008 - annualreviews.org
The surfaces of aqueous solutions are traditionally viewed as devoid of inorganic ions.
Molecular simulations and surface-selective spectroscopic techniques show, however, that …
Molecular simulations and surface-selective spectroscopic techniques show, however, that …
Hydrophobicity of proteins and interfaces: Insights from density fluctuations
SN Jamadagni, R Godawat… - Annual review of chemical …, 2011 - annualreviews.org
Macroscopic characterizations of hydrophobicity (eg, contact angle measurements) do not
extend to the surfaces of proteins and nanoparticles. Molecular measures of hydrophobicity …
extend to the surfaces of proteins and nanoparticles. Molecular measures of hydrophobicity …